Insampaedok-San Extract Exerts an Immune-Enhancing Effect through NF-κB p65 Pathway Activation.
Insampaedok-san (IS) has traditionally been prescribed as a medication for cold-related symptoms in Northeast Asia, including Korea and China. In this study, we focused on elucidating the molecular mechanism underlying the immunomodulatory activity of IS water extract (ISE) in macrophages. ISE signi...
| Publicado en: | BioMed Research International pp. 1 - 14 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
9/22/2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=172447518&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 172447518 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 9/22/2023 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 172447518 172447518 172447518 10.1155/2023/5458504 172447518 ppf: 1 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Insampaedok-San Extract Exerts an Immune-Enhancing Effect through NF-κB p65 Pathway Activation. aug: au: Huh, Gyuwon Oh, Youngse Jeon, Youngsic Kang, Ki Sung Kim, Su Nam Jung, Sang Hoon Kim, Seung Hyun Kim, Young-Joo affil: Natural Product Research Center, Korea Institute of Science and Technology, Gangneung 25451, Republic of Korea sug: subj: Medicine, Chinese Traditional Common Cold Drug Therapy Plant Extracts Therapeutic Use Plant Extracts Pharmacodynamics Immunologic Factors Pharmacodynamics Signal Transduction Drug Effects NF-kappa B Drug Effects Treatment Outcomes In Vitro Studies South Korea China Nitric Oxide Blood Cox-2 Inhibitors Drug Effects Nitric Oxide Synthases Drug Effects Network Pharmacology Dinoprostone Drug Effects Macrophages Mass Spectrometry Methods Chromatography, High Pressure Liquid Methods RNA, Messenger Drug Effects Tumor Necrosis Factor Drug Effects Interleukins Drug Effects Phosphorylation Drug Effects Funding Source ab: Insampaedok-san (IS) has traditionally been prescribed as a medication for cold-related symptoms in Northeast Asia, including Korea and China. In this study, we focused on elucidating the molecular mechanism underlying the immunomodulatory activity of IS water extract (ISE) in macrophages. ISE significantly enhanced the levels of nitric oxide (NO) and prostaglandin E2 (PGE2) by increasing the expression of inducible NO synthase and cyclooxygenase-2 (COX-2) in a dose-dependent manner. ISE, which consists of many herbs, contains a large number of active compounds whose pharmacological targets and mechanisms are complicated. Therefore, network pharmacology analysis was used to predict the potential key components, targets, and mechanisms of ISE as immunomodulators. Subsequently, the network pharmacology results were validated experimentally. Seven key components were identified through HPLC-QTOF-MS. As predicted by the network pharmacology analysis, ISE increased the mRNA expression of Tnf and Il6. Furthermore, ISE increased the phosphorylation, nuclear translocation, and transcriptional activity of the p65 subunit of the nuclear factor-κB (NF-κB) signaling pathway. In contrast, rapamycin, an NF-κB inhibitor, suppressed the ISE-induced mRNA expression of Tnf and Il6. In conclusion, ISE is an immune activator that can elevate the production of NO, PGE2, and proinflammatory cytokines mediated by NF-κB signaling. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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