Evaluation of TRAF3IP2 Gene Expression in Brain Tumor Tissue of Patients with Glioblastoma Multiforme in Comparison to Non-Tumoral Brain Tissue.

Background: Glioblastoma, not otherwise specified (NOS), is the most common primary malignant brain tumor. The TRAF3IP2 gene is an upstream regulator responsible for activating multiple proinflammatory pathways that could influence tumor size, angiogenesis, aggressiveness, and metastasis. In the pre...

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Publicado en:Middle East Journal of Cancer Vol. 14; no. 4; pp. 543 - 550
Autores principales: Mahzouni, Parvin, Seddighin, Roya, Ghasroldasht, Mohammad Mousaei
Formato: research tables/charts Journal Article
Publicado: Middle East Journal of Cancer Oct2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Oct2023
      vid: 14
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      pid: 67247
      pub: Middle East Journal of Cancer
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        10.30476/mejc.2023.96063.1800
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        atl: Evaluation of TRAF3IP2 Gene Expression in Brain Tumor Tissue of Patients with Glioblastoma Multiforme in Comparison to Non-Tumoral Brain Tissue.
      aug:
        au:
          Mahzouni, Parvin
          Seddighin, Roya
          Ghasroldasht, Mohammad Mousaei
        affil: Department of Pathology, School of Medicine, Al-Zahra Hospital, Isfahan University of Medical Sciences, Isfahan, Iran
      sug:
        subj:
          Glioma Physiopathology
          Gene Expression Evaluation
          Tissue Analysis
          Human
          Male
          Female
          Cancer Patients
          Funding Source
          Comparative Studies
          Case Control Studies
          Biopsy
          RNA
          Polymerase Chain Reaction Methods
          Descriptive Statistics
          Sex Factors
          Male
          Female
      ab: Background: Glioblastoma, not otherwise specified (NOS), is the most common primary malignant brain tumor. The TRAF3IP2 gene is an upstream regulator responsible for activating multiple proinflammatory pathways that could influence tumor size, angiogenesis, aggressiveness, and metastasis. In the present study, we aimed to investigate and assess the TRAF3IP2 gene expression in brain tumor tissue of patients with glioblastoma, NOS and compare it with non-neoplastic brain tissue. Method: In this case-control study, biopsies were obtained from 15 surgically glioblastoma, NOS removed block samples and 15 non-neoplastic brain tissue samples containing normal white and gray matter as controls. Ribonucleic acid (RNA) was isolated and reverse-transcribed to complementary DNA (cDNA). Quantitative polymerase chain reaction (qPCR) was then carried out to measure TRAF3IP2 gene expression. Results: We evaluated data from 30 cases, divided into two groups: case (N = 15) and control (N = 15). Based on our data, the expression of the TRAF3IP2 gene was 6.95 ± 0.65 times higher in glioblastoma multiforme tissue compared with controls (P < 0.05). We also found no significant difference in TRAF3IP2 gene expression between genders (P = 0.452), and there was no significant correlation between TRAF3IP2 gene expression and age (P = 0.745). Conclusion: The expression of the TRAF3IP2 gene was almost seven times higher in glioblastoma, NOS brain tissue compared with normal brain samples. This finding could have significant clinical and therapeutic implications.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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