The study of bioavailability and endogenous circadian rhythm of menaquinone-7, a form of vitamin K2, in healthy subjects.

Menaquinone-7 (MK-7), a multipotent vitamin K2, possesses a wide range of biological activities, a precise curative effect and excellent safety. A simple and rapid LC-APCI-MS/MS method for the determination of MK-7 in human plasma with single liquid–liquid extraction (LLE) extraction and 4·5-min ana...

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Publicado en:British Journal of Nutrition Vol. 130; no. 11; pp. 1885 - 1898
Autores principales: Du, Fan, Yan, Min, Duan, Lili, Xie, Guolong, Yao, Xiuhua, Hu, Wenjing, Liu, Yu, Meng, Min, Chen, Jiepeng, Shao, Di
Formato: pictorial research tables/charts Journal Article
Publicado: Cambridge University Press 12/14/2023
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: British Journal of Nutrition
      issn: 00071145
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      dt: 12/14/2023
      vid: 130
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      pub: Cambridge University Press
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        10.1017/S0007114523001034
        173476440
      ppf: 1885
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        atl: The study of bioavailability and endogenous circadian rhythm of menaquinone-7, a form of vitamin K2, in healthy subjects.
      aug:
        au:
          Du, Fan
          Yan, Min
          Duan, Lili
          Xie, Guolong
          Yao, Xiuhua
          Hu, Wenjing
          Liu, Yu
          Meng, Min
          Chen, Jiepeng
          Shao, Di
        affil: Chonggang General Hospital, Chongqing, People's Republic of China
      sug:
        subj:
          Vitamin K Pharmacokinetics
          Biological Availability
          Circadian Rhythm
          Human
          Albumins
          Male
          Vitamin K Administration and Dosage
          Pilot Studies
          Funding Source
          Male
      ab: Menaquinone-7 (MK-7), a multipotent vitamin K2, possesses a wide range of biological activities, a precise curative effect and excellent safety. A simple and rapid LC-APCI-MS/MS method for the determination of MK-7 in human plasma with single liquid–liquid extraction (LLE) extraction and 4·5-min analysis time has been developed and validated. Four per cent bovine serum albumin (BSA) was used as surrogate matrix for standard curves and endogenous baseline subtraction. This method was reproducible and reliable and was used to analyse of MK-7 in human plasma. The endogenous circadian rhythm and bioavailability of MK-7 were investigated in two randomised single-dose, open, one-way clinical trials (Study I and Study II). A total of five healthy male subjects were enrolled in Study I and 12 healthy male subjects in Study II. Single-dose (1 mg) of MK-7 was given to each subject under fasting condition, and all eligible subjects were given a restricting VK2 diet for 4 d prior to drug administration and during the trial. The experiment results of Study I demonstrated that endogenous MK-7 has no circadian rhythm in individuals. Both studies showed MK-7 are absorbed with peak plasma concentrations at about 6 h after intake and has a very long half-life time.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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