Pattern of Primary Resistance of Helicobacter pylori to Clarithromycin among Pediatric Patients from North-Eastern Romania.
Background: Helicobacter pylori antibiotic resistance has increased worldwide and affects the effectiveness of current therapies. The recommended first-line empiric treatment should be tailored to the local clarithromycin resistance rate. This study aimed to determine the pediatric patient profile a...
| Publicado en: | Children Vol. 10; no. 11; pp. 1752 - 1763 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
MDPI
Nov2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=173825280&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 173825280 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 22279067 LW6K jtl: Children issn: 22279067 maglogo: N pubinfo: dt: Nov2023 vid: 10 iid: 11 pid: 97109 pub: MDPI artinfo: ui: 173825280 173825280 173825280 10.3390/children10111752 173825280 ppf: 1752 ppct: 11 formats: tig: atl: Pattern of Primary Resistance of Helicobacter pylori to Clarithromycin among Pediatric Patients from North-Eastern Romania. aug: au: Rosu, Oana-Maria Gimiga, Nicoleta Popescu, Roxana Ioniuc, Ileana Rusu, Carmen Daniela Clipa, Tatiana Florea, Diana-Maria Pleșca, Doina-Anca Nemtoi, Alexandru Tataranu, Elena Stefanescu, Gabriela Diaconescu, Smaranda affil: Doctoral School, "George Emil Palade" University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 38 Gheorghe Marinescu Str., 540139 Targu Mures, Romania sug: subj: Drug Resistance, Microbial Evaluation Helicobacter Pylori Helicobacter Infections Drug Therapy Clarithromycin Therapeutic Use Pediatric Care Human Male Female Child Adolescence Romania Biopsy Methods Reverse Transcriptase Polymerase Chain Reaction Sex Factors Prospective Studies Descriptive Statistics T-Tests Chi Square Test Pearson's Correlation Coefficient Data Analysis Software Quantitative Studies Child: 6-12 years Adolescent: 13-18 years Male Female ab: Background: Helicobacter pylori antibiotic resistance has increased worldwide and affects the effectiveness of current therapies. The recommended first-line empiric treatment should be tailored to the local clarithromycin resistance rate. This study aimed to determine the pediatric patient profile and rate of clarithromycin resistance for patients diagnosed with Helicobacter pylori by gastric biopsy. Methods: We studied 84 positive gastric samples for Helicobacter pylori. Positive results were confirmed by a rapid urease test and histopathological examination, with the type of gastritis established according to the Sydney System. Gastric biopsy samples were stored in RNA saver. Clarithromycin resistance was determined by a real-time polymerase chain reaction-based molecular assay after RNA-DNA extraction. Results: Of the 84 biopsy samples analyzed, 35 (41.6%) were resistant to clarithromycin. Clarithromycin resistance was found mainly in girls (80%) with a mean age of 15 years (range 6–17 years). The history of prior exposure to clarithromycin was 91.6%. The concordance between the histopathological examination and the PCR test was 100%. Conclusions: One in 2.4 children infected with Helicobacter pylori had a strain resistant to clarithromycin. This resistant strain may be a reason for treatment failure in Romanian children, yet this is uninvestigated. The high rate of bacterial resistance to this antibiotic among children indicates the need for susceptibility testing before therapy. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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