Occurrence, molecular diversity and pathogenicity of Acanthamoeba spp. isolated from aquatic environments of Northeastern Brazil.
Acanthamoeba is a free-living amoeba (FLA) that is ubiquitous in nature and can cause serious pathologies in humans. This protozoan has been detected in several environmental sources, such as soil, water, and swimming pools. The aim of this study was to evaluate the occurrence and molecular diversit...
| Publicado en: | International Journal of Environmental Health Research Vol. 33; no. 12; pp. 1686 - 1697 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Dec2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174203859&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174203859 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09603123 57L jtl: International Journal of Environmental Health Research issn: 09603123 maglogo: Y pubinfo: dt: Dec2023 vid: 33 iid: 12 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 174203859 158788965 174203859 174203859 10.1080/09603123.2022.2117280 174203859 ppf: 1686 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Occurrence, molecular diversity and pathogenicity of Acanthamoeba spp. isolated from aquatic environments of Northeastern Brazil. aug: au: Matos de Oliveira, Yrna Lorena Lima, Erica Tirzah Santos Rott, Marilise Brittes Fernandes, Roberta Pereira Miranda Jain, Sona de Aragão Batista, Marcus Vinicius Santana Dolabella, Silvio affil: Laboratory of Entomology and Tropical Parasitology, Department of Morphology, University of Sergipe, São Cristóvão, Sergipe, Brazil sug: subj: Protozoa Brazil Natural Environment Water Microbiology Virulence Genotype Environmental Health Human Brazil Funding Source Cell Culture Techniques Descriptive Statistics Protozoan Infections Prevention and Control ab: Acanthamoeba is a free-living amoeba (FLA) that is ubiquitous in nature and can cause serious pathologies in humans. This protozoan has been detected in several environmental sources, such as soil, water, and swimming pools. The aim of this study was to evaluate the occurrence and molecular diversity of Acanthamoeba spp. in aquatic environments of the state of Sergipe, northeastern Brazil, and to determine the pathogenic potential of the isolated samples. A total of 138 samples were collected from 69 aquatic environments and, after cell culture, 74% of the samples were positive for FLA, 47% belonging to the genus Acanthamoeba. Genotypic analysis was performed using the primers JDP1 and JDP2, confirming distinct Acanthamoeba genotypes: 18 (75%) isolates belonging to genotype T4, two (8%) to T3, and one isolate (4%) to genotype T5. Tests carried out to analyze the pathogenic potential showed that 11 isolates could grow at 0.5 M mannitol concentration and seven isolates supported hyperosmolarity. In the thermotolerance test, two isolates grew at 37°C. These results confirm the presence and the pathogenic potential of FLA of the genus Acanthamoeba in aquatic environments of the municipalities of Sergipe. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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