The effectiveness of therapeutic ultrasound to the mechanically damaged chondrocyte culture.
This study aimed to evaluate the efficacy of low intensity (0.1–0.8 Watt/cm2) pulsed ultrasound on chondrocyte cell proliferation and migration. Low-intensity pulsed ultrasound (frequency 3 MHz, duty cycle 25%, and pulse repetition frequency 150 Hz) for 5 minutes at different spatial average intensi...
| Published in: | Physiotherapy Theory & Practice Vol. 40; no. 1; pp. 21 - 31 |
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| Main Authors: | , , , , |
| Format: | clinical trial pictorial research tables/charts Journal Article |
| Published: |
Taylor & Francis Ltd
Jan2024
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174521840&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174521840 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09593985 BCJ jtl: Physiotherapy Theory & Practice issn: 09593985 maglogo: Y pubinfo: dt: Jan2024 vid: 40 iid: 1 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 174521840 158264354 174521840 174521840 10.1080/09593985.2022.2107466 174521840 ppf: 21 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: The effectiveness of therapeutic ultrasound to the mechanically damaged chondrocyte culture. aug: au: Sabanci, Serkan Sabanci, Sercan Sendur, Omer Faruk Sakarya, Serhan Yilmaz, Ozgenur affil: Department of Physical Medicine and Rehabilitation, Adnan Menderes University School of Medicine, Aydin, Turkey sug: subj: Chondrocytes Ultrasonography Ultrasonic Therapy Methods Cell Proliferation Drug Effects Chondrocytes Pathology Culture Chondrocytes Physiology Cell Movement Drug Effects Treatment Outcomes Evaluation Human Descriptive Statistics Funding Source Comparative Studies Treatment Outcomes Antirheumatic Agents Therapeutic Use Osteoarthritis Drug Therapy Post Hoc Analysis Clinical Trials ab: This study aimed to evaluate the efficacy of low intensity (0.1–0.8 Watt/cm2) pulsed ultrasound on chondrocyte cell proliferation and migration. Low-intensity pulsed ultrasound (frequency 3 MHz, duty cycle 25%, and pulse repetition frequency 150 Hz) for 5 minutes at different spatial average intensities was applied on chondrocyte cells. First, the optimum dose with significantly increased proliferation was determined to be 0.5 W/cm2 for 5 minutes of duration. Then, 0.5 W/cm2 ultrasound intensity was applied for durations of 2.5, 5, 7.5, and 10 minutes, and healing was assessed by monitoring cell migration and proliferation. At the intensity of 0.5 W/cm2 48 hours after the application, a statistically significant increase in proliferation (p =.0089) was observed in chondrocyte cells compared to the control group. Proliferation was analyzed at 4, 8, 24, and 48 hours after applying 0.5 W/cm2 ultrasound for durations of 2.5, 5, 7.5, and 10 minutes. Statistically significant increases were observed at 4 hours (p =.009), 8 hours (p =.032), 24 hours (p =.0084), and 48 hours (p =.00098) with 10 minutes of exposure. For 7.5 min of exposure duration, significant increases were observed at 48 hours (p =.045). In migration for all parameters, no statistically significant improvement (p >.05) was observed. It was shown that low intensity pulsed ultrasound induces cartilage cell proliferation; therefore, it may have a disease-modifying effect in the treatment of osteoarthritis. However, no positive effect was observed on cartilage cell migration. pubtype: Academic Journal doctype: clinical trial pictorial research tables/charts Journal Article ougenre: Unknown language: English refInfo: holdings: @attributes: islocal: N |
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