Secretome of stem cells from human exfoliated deciduous teeth (SHED) and its extracellular vesicles improves keratinocytes migration, viability, and attenuation of H2O2‐induced cytotoxicity.
Therapies for wound healing using the secretome and extracellular vesicles (EVs) of mesenchymal stem/stromal cells have been shown to be successful in preclinical studies. This study aimed to characterise the protein content of the secretome from stem cells from human exfoliated deciduous teeth (SHE...
| Published in: | Wound Repair & Regeneration Vol. 31; no. 6; pp. 827 - 842 |
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| Main Authors: | , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Nov/Dec2023
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174563082&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174563082 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov/Dec2023 vid: 31 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 174563082 174057446 174563082 174563082 10.1111/wrr.13131 174563082 ppf: 827 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Secretome of stem cells from human exfoliated deciduous teeth (SHED) and its extracellular vesicles improves keratinocytes migration, viability, and attenuation of H2O2‐induced cytotoxicity. aug: au: Bastidas, Juliana Girón Maurmann, Natasha Scholl, Juliete Nathali Weber, Augusto Ferreira Silveira, Raíssa Padilha Figueiró, Fabricio Stimamiglio, Marco Augusto Marcon, Bruna Correa, Alejandro Pranke, Patricia affil: Hematology & Stem Cell Laboratory, Faculty of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre Rio Grande do Sul,, Brazil sug: subj: Stem Cells Metabolism Culture Media Metabolism Keratinocytes Extracellular Vesicles Metabolism Tooth, Deciduous Cell Movement Cell Physiology Proteomics Secretome Vascular Endothelial Growth Factor A Metabolism Wound Healing Hydrogen Peroxide Human Male Female In Vitro Studies Apoptosis Gene Expression Reverse Transcriptase Polymerase Chain Reaction Methods Descriptive Statistics Comparative Studies Fluorescent Antibody Technique Microscopy Methods Cytokines Funding Source Male Female ab: Therapies for wound healing using the secretome and extracellular vesicles (EVs) of mesenchymal stem/stromal cells have been shown to be successful in preclinical studies. This study aimed to characterise the protein content of the secretome from stem cells from human exfoliated deciduous teeth (SHED) and analyse the in vitro effects of SHED‐conditioned medium (SHED‐CM) and SHED extracellular vesicles (SHED‐EVs) on keratinocytes. EVs were isolated and characterised. The keratinocyte viability and migration of cells treated with SHED‐EVs and conditioned medium (CM) were evaluated. An HaCaT apoptosis model induced by H2O2 in vitro was performed with H2O2 followed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) and live/dead assays. Finally, the expression of vascular endothelial growth factor (VEGF) in keratinocytes treated with secretome and EVs was evaluated by immunofluorescence staining and confirmed with RT‐qPCR. SHED‐EVs revealed a cup‐shaped morphology with expression of the classical markers for exosomes CD9 and CD63, and a diameter of 181 ± 87 nm. The internalisation of EVs by HaCaT cells was confirmed by fluorescence microscopy. Proteomic analysis identified that SHED‐CM is enriched with proteins related to stress response and development, including cytokines (CXCL8, IL‐6, CSF1, CCL2) and growth factors (IGF2, MYDGF, PDGF). The results also indicated that 50% CM and 0.4–0.6 μg/mL EVs were similarly efficient for improving keratinocyte viability, migration, and attenuation of H2O2‐induced cytotoxicity. Additionally, expression of VEGF on keratinocytes increased when treated with SHED secretome and EVs. Furthermore, VEGF gene expression in keratinocytes increased significantly when treated with SHED secretome and EVs. Both SHED‐CM and SHED‐EVs may therefore be promising therapeutic tools for accelerating re‐epithelialization in wound healing. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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