Curcumin Induces Apoptosis by Suppressing XRCC4 Expression in Hepatocellular Carcinoma.
Curcumin is a chemical with various pharmacological activities used for cancer treatment. It inhibits hepatocellular carcinoma (HCC) by inducing apoptosis. Here, the mechanism underlying the effect of curcumin on the apoptosis of HCC cells was studied. Cell counting kit-8 and plate cloning assays we...
| Publicado en: | Nutrition & Cancer Vol. 75; no. 10; pp. 1958 - 1968 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174566759&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174566759 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2023 vid: 75 iid: 10 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 174566759 173277006 174566759 174566759 10.1080/01635581.2023.2274132 174566759 ppf: 1958 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Curcumin Induces Apoptosis by Suppressing XRCC4 Expression in Hepatocellular Carcinoma. aug: au: Shixin Wang Wangqiu Wu Yaxin Liu Enjia Xing Jingtong Jiao Ling Li Jingmin Li Dong Wang affil: Department of Histology and Embryology, Binzhou Medical University, Yantai, Shandong, China. sug: subj: Carcinoma, Hepatocellular Drug Therapy Curcumin Pharmacodynamics Apoptosis Oncogenes Drug Effects Gene Expression Drug Effects Treatment Outcomes Animal Studies Mice Biological Assay Cell Proliferation Immunohistochemistry Carcinoma, Hepatocellular Prognosis Comparative Studies In Vivo Studies In Vitro Studies Funding Source ab: Curcumin is a chemical with various pharmacological activities used for cancer treatment. It inhibits hepatocellular carcinoma (HCC) by inducing apoptosis. Here, the mechanism underlying the effect of curcumin on the apoptosis of HCC cells was studied. Cell counting kit-8 and plate cloning assays were used to assess the proliferation of HCC cells, and acridine orange/ethidium bromide and Annexin V/PI staining were used to analyze their apoptosis. HCC xenograft tumor models were established to validate anti-cancer effects of curcumin. Expression levels of XRCC4 protein in tumor tissues were assessed by immunohistochemistry. Correlation between XRCC4 expression and the prognosis of patients with HCC was analyzed by integrating publicly available gene expression data. Curcumin inhibited HCC cells proliferation in a dose-dependent manner. Compared with the control group, curcumin significantly promoted the apoptosis of HCC cells in vitro and in vivo. Immunohistochemical analysis revealed that curcumin downregulated XRCC4 expression levels in HCC tissues. Prognosis of HCC patients with high XRCC4 expression was poorer than that of patients with low XRCC4 expression. Therefore, curcumin exerts anti-cancer effects by inhibiting cell proliferation and promoting cell apoptosis in HCC. This may be due to curcumin interference in the repair process of the nonhomologous DNA terminal link of HCC cells by downregulating XRCC4 expression. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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