Proliferation and apoptosis after whole-body irradiation: longitudinal PET study in a mouse model.
Purpose: A reliable method for regional in vivo imaging of radiation-induced cellular damage would be of great importance for the detection of therapy-induced injury to healthy tissue and the choice of adequate treatment of radiation emergency patients in both civilian and military events. This stud...
| Publicado en: | European Journal of Nuclear Medicine & Molecular Imaging Vol. 51; no. 2; pp. 395 - 405 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jan2024
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174658092&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174658092 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16197070 NPC jtl: European Journal of Nuclear Medicine & Molecular Imaging issn: 16197070 maglogo: N pubinfo: dt: Jan2024 vid: 51 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 174658092 172794679 10.1007/s00259-023-06430-x 174658092 ppf: 395 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Proliferation and apoptosis after whole-body irradiation: longitudinal PET study in a mouse model. aug: au: Meindl, Maria Bläske, Alexandra Steiger, Katja Lindner, Simon Lindheimer, Felix Lauber, Kirsten Brix, Nikko von Ungern-Sternberg, Barbara Oos, Rosel Palumbo, Giovanna Böning, Guido Schüle, Simone Majewski, Matthäus Port, Matthias Ziegler, Sibylle Bartenstein, Peter affil: Department of Nuclear Medicine, LMU University Hospital, LMU Munich, Munich, Germany sug: ab: Purpose: A reliable method for regional in vivo imaging of radiation-induced cellular damage would be of great importance for the detection of therapy-induced injury to healthy tissue and the choice of adequate treatment of radiation emergency patients in both civilian and military events. This study aimed to investigate in a mouse model if positron emission tomography (PET) imaging with proliferation and apoptosis markers is potentially suitable for this purpose. Methods: Four groups, including twenty mice (wild-type C57BL/6) each, were whole-body irradiated with 0 Gy, 0.5 Gy, 1 Gy, and 3 Gy and examined by PET over a six-month period at defined time points. 3'-[18F]fluoro-3'-deoxythymidine ([18F]FLT) and 2-(5-[18F]fluoropentyl)-2-methyl malonic acid ([18F]ML-10) were used to visualise proliferation and apoptosis. Regional standard uptake values were compared with respect to irradiation dose over time. Histologic data and peripheral blood cell values were correlated with the PET results. Results: The hematopoietic bone marrow showed a significantly increased [18F]FLT signal at early time points after radiation exposure (day 3 and day 7). This correlated with blood parameters, especially leukocytes, and histological data. A significantly increased [18F]FLT signal also occurred in the gastrointestinal tract and thymus at early time points. An increased [18F]ML-10 signal related to irradiation doses was observed in the bone marrow on day 8, but there was a high variability of standard uptake values and no correlation with histological data. Conclusion: [18F]FLT showed potential to visualise the extent, regional distribution and recovery from radiation-induced cellular damage in the bone marrow, gastrointestinal tract and thymus. The potential of [18F]FLT imaging to assess the extent of bone marrow affected by irradiation might be especially useful to predict the subsequent severity of hematopoietic impairment and to adapt the therapy of the bone marrow reserve. [18F]ML-10 PET proved to be not sensitive enough for the reliable detection of radiation induced apoptosis. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|