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Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia.

We present a label-free microfluidic chip for the segregation of circulating leukemia cells (CLCs) from blood samples, with a focus on its clinical applications in Acute Myeloid Leukemia (AML). The microfluidic chip achieved an approximate capture efficiency of 92%. The study analyzed a comprehensiv...

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Published in:Biomedical Microdevices Vol. 26; no. 1; pp. 1 - 14
Main Authors: Ouyang, Dongfang, Ye, Ningxin, Jiang, Yue, Wang, Yiyang, Hu, Lina, Chao, Shuen, Yarmush, Martin, Tuner, Memet, Li, Yonghua, Tang, Bin
Format: Journal Article
Published: Springer Nature Mar2024
Online Access:View this record in EBSCOhost
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s10544-023-00687-7
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        atl: Label-free microfluidic chip for segregation and recovery of circulating leukemia cells: clinical applications in acute myeloid leukemia.
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        au:
          Ouyang, Dongfang
          Ye, Ningxin
          Jiang, Yue
          Wang, Yiyang
          Hu, Lina
          Chao, Shuen
          Yarmush, Martin
          Tuner, Memet
          Li, Yonghua
          Tang, Bin
        affil: Center for Engineering in Medicine and Surgery, Massachusetts General Hospital, Harvard Medical School, 02129, Charlestown, MA, USA
      sug:
      ab: We present a label-free microfluidic chip for the segregation of circulating leukemia cells (CLCs) from blood samples, with a focus on its clinical applications in Acute Myeloid Leukemia (AML). The microfluidic chip achieved an approximate capture efficiency of 92%. The study analyzed a comprehensive set of 66 blood specimens from AML patients in different disease stages, including newly diagnosed and relapsing cases, patients in complete remission, and those in partial remission. The results showed a significant difference in CLC counts between active disease stages and remission stages (p < 0.0001), with a proposed threshold of 5 CLCs to differentiate between the two. The microfluidic chip exhibited a sensitivity of 95.4% and specificity of 100% in predicting disease recurrence. Additionally, the captured CLCs were subjected to downstream molecular analysis using droplet digital PCR, allowing for the identification of genetic mutations associated with AML. Comparative analysis with bone marrow aspirate processing by FACS demonstrated the reliability and accuracy of the microfluidic chip in tracking disease burden, with highly agreement results obtained between the two methods. The non-invasive nature of the microfluidic chip and its ability to provide real-time insights into disease progression make it a promising tool for the proactive monitoring and personalized patient care of AML.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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