Vitamin D3 supplementation shapes the composition of gut microbiota and improves some obesity parameters induced by high-fat diet in mice.
Purpose: Individuals with vitamin D (VD) insufficiency have a greater tendency to develop obesity and have increased systemic inflammation. Gut microbiota are involved in the regulation of host inflammation and energy metabolism, which plays a role in the pathogenesis of obesity. Thus, we aimed to e...
| Publicado en: | European Journal of Nutrition Vol. 63; no. 1; pp. 155 - 173 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Feb2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174918769&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174918769 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Feb2024 vid: 63 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 174918769 172271701 174918769 174918769 10.1007/s00394-023-03246-1 174918769 ppf: 155 ppct: 18 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Vitamin D3 supplementation shapes the composition of gut microbiota and improves some obesity parameters induced by high-fat diet in mice. aug: au: Xiang, Lian Du, Tingwan Zhang, Jingjing Zhang, Yuanfan Zhou, Yanqiu Zhao, Yueying Zhou, Yong Ma, Ling affil: https://ror.org/00g2rqs52 Department of Nutrition and Food Hygiene, School of Public Health, Southwest Medical University, Luzhou, China sug: subj: Gut Microbiota Metabolism Obesity Prevention and Control Dietary Fiber Administration and Dosage Obesity Drug Therapy Cholecalciferol Therapeutic Use Diet Adverse Effects Treatment Outcomes Dietary Supplements Cholecalciferol Pharmacodynamics Animal Studies Rats Descriptive Statistics RNA Analysis Body Weight Drug Effects Lipids Drug Effects Tumor Necrosis Factor Drug Effects Interleukins Drug Effects Bacteria Drug Effects Funding Source ab: Purpose: Individuals with vitamin D (VD) insufficiency have a greater tendency to develop obesity and have increased systemic inflammation. Gut microbiota are involved in the regulation of host inflammation and energy metabolism, which plays a role in the pathogenesis of obesity. Thus, we aimed to evaluate the effects of different doses of VD3 on body weight, serum lipids, inflammatory factors, and intestinal barrier function in obese mice and to explore the regulatory effect of VD3 on gut microbiota in obese mice. Methods: Male C57BL/6 J mice received a normal chow diet (NCD, 10% fat) or high-fat diet (HFD, 60% fat) to induce obesity within 10 weeks. Then, HFD mice were supplemented with 5650, 8475, or 11,300 IU VD3/kg diet for 8 weeks. Finally, 16 s rRNA analysis was performed to analyze gut microbiota composition in cecal contents. In addition, body weight, serum lipids, inflammatory factors, and intestinal barrier function were analyzed. Results: VD3 supplementation reduced body weight and the levels of TG, TC, HDL-C, TNF-α, IL-1β and LPS, and increased ZO-1 in HFD-fed mice. Moreover, it increased α-diversity, reduced F/B ratio and altered microbiota composition by increasing relative abundance of Bacteroidetes, Proteobacteria, Desulfovibrio, Dehalobacterium, Odoribacter, and Parabacteroides and reducing relative abundance of Firmicutes and Ruminococcus. There were significant differences between HFD and NCD groups in several metabolic pathways, including endotoxin biosynthesis, tricarboxylic acid cycle, lipid synthesis and metabolism, and glycolysis. Conclusions: Low, medium, and high doses of VD3 inhibited weight gain, reduced levels of blood lipids and inflammatory factors, and improved endotoxemia and gut barrier function in obese mice. It also increased the α-diversity of gut microbiota in obese mice and reduced the relative abundance of some intestinal pathogenic bacteria, increased the relative abundance of some beneficial bacteria, and corrected the intestinal flora disorder of obese mice, with the low- and high-dose groups showing better effects than the medium-dose group. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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