Bioguided fractionation of procyanidin B2 as potent anti coxsackie virus B and Herpes simplex from cypress (Cupressus sempervirens L.).
The methanolic extracts of cypress (Cupressus sempervirens L.) collected at three phenological stages were evaluated for their cytotoxicity on Vero cells by MTT test as well as on Herpes simplex (HSV-2) and coxsackie (CVB-3) viruses by plaque reduction assay. The methanolic extract exhibited the hig...
| Publicado en: | International Journal of Environmental Health Research Vol. 34; no. 1; pp. 191 - 201 |
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| Autores principales: | , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jan2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=174973571&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 174973571 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09603123 57L jtl: International Journal of Environmental Health Research issn: 09603123 maglogo: Y pubinfo: dt: Jan2024 vid: 34 iid: 1 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 174973571 159757827 174973571 174973571 10.1080/09603123.2022.2137475 174973571 ppf: 191 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Bioguided fractionation of procyanidin B2 as potent anti coxsackie virus B and Herpes simplex from cypress (Cupressus sempervirens L.). aug: au: Rguez, Safa Hammami, Majdi Aidi Wannes, Wissem Hamrouni Sellami, Ibtissem affil: Laboratory of Medicinal and Aromatic Plants, Centre of Biotechnology of Borj Cedria, Hammam-Lif, Tunisia sug: subj: Herpes Simplex Drug Therapy Coxsackievirus Infections Drug Therapy Plant Extracts Analysis Plants, Medicinal Therapeutic Use Plant Extracts Pharmacodynamics Treatment Outcomes Flavonoids Antioxidants Data Analysis Software Chromatography, High Pressure Liquid Chromatography, Thin Layer Antiviral Agents Phytochemicals Coxsackieviruses ab: The methanolic extracts of cypress (Cupressus sempervirens L.) collected at three phenological stages were evaluated for their cytotoxicity on Vero cells by MTT test as well as on Herpes simplex (HSV-2) and coxsackie (CVB-3) viruses by plaque reduction assay. The methanolic extract exhibited the highest cytotoxicity against HSV-2 (IC50 = 20.40 µg/mL) and CVB-3 (IC50 = 47.50 µg/mL) at the flowering stage. This extract also exhibited a virucidal action both during the entry of viruses and the release of newly formed virions. The methanolic extract bioguided purification showed that the ethyl-acetate fraction was responsible for virucidal activity. This fraction was endowed with more important selectivity index of 8.15 for HSV-2 and 4.40 for CVB-3. The ethyl acetate fraction was subjected to thin layer chromatography fractionation and identification by HPLC-DAD-ESI-MSn. Results showed that the condensed tannin procyanidin B2 was identified for the first time responsible of the antiviral activity of cypress. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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