The transcript NR 134251.1 of lncRNA APTR with an opposite function to all transcripts inhibits proliferation and induces apoptosis by regulating proliferation and apoptosis-related genes.

Arsenic (As) exposure has been a global public health concern for hundreds of millions worldwide. LncRNA APTR (Alu-mediated p21 transcriptional regulator) plays an essential role in tumor growth and development. However, its function in arsenic-induced toxicological responses is still unknown. In th...

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Publicado en:Human & Experimental Toxicology Vol. 42; pp. 1 - 11
Autores principales: Yu, Jinyi, Li, Shuting, Shen, Simin, Zhou, Qian, Yin, Jinyao, Zhao, Ruihuan, Tan, Jingwen, Jiang, Chenglan, He, Yuefeng
Formato: pictorial research tables/charts Journal Article
Publicado: Sage Publications Inc. Jan-Dec2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jan-Dec2023
      vid: 42
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      pub: Sage Publications Inc.
      place: Thousand Oaks, California
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        10.1177/09603271221150247
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        atl: The transcript NR 134251.1 of lncRNA APTR with an opposite function to all transcripts inhibits proliferation and induces apoptosis by regulating proliferation and apoptosis-related genes.
      aug:
        au:
          Yu, Jinyi
          Li, Shuting
          Shen, Simin
          Zhou, Qian
          Yin, Jinyao
          Zhao, Ruihuan
          Tan, Jingwen
          Jiang, Chenglan
          He, Yuefeng
        affil: School of Public Health, 71240 Kunming Medical University, Kunming, China
      sug:
        subj:
          Transcription Factors Physiology
          RNA Physiology
          Proteins Physiology
          Cell Proliferation Drug Effects
          Apoptosis Drug Effects
          Genes Drug Effects
          Arsenic Adverse Effects
          Sodium Compounds Adverse Effects
          Human
          Funding Source
          Genes, Tumor Suppressor
          Caspases
          Gene Expression
      ab: Arsenic (As) exposure has been a global public health concern for hundreds of millions worldwide. LncRNA APTR (Alu-mediated p21 transcriptional regulator) plays an essential role in tumor growth and development. However, its function in arsenic-induced toxicological responses is still unknown. In this study, we found that the expressions of all transcripts and the transcript NR 134251.1 of APTR were increased in a dose-dependent manner in 16HBE cells treated with sodium arsenite (NaAsO2). Silencing the transcript NR 134251.1 of APTR inhibited cell proliferation and induced apoptosis. However, silencing all transcripts of APTR had the opposite function to the transcript NR 134251.1. Then we examined the protein level of the proliferation and apoptosis-related genes after silencing the transcript NR 134251.1 of APTR. The results showed that silencing the transcript NR 134251.1 of APTR up-regulated the expression of transcription factor E2F1 and regulated its downstream genes involved in proliferation and apoptosis, including p53, phospho-p53-S392, phospho-p53-T55, p21, Cyclin D1, PUMA, Fas, Bim, BIK, Caspase-3, Caspase-7, and Cyt-c. In conclusion, arsenic induced APTR expression and the transcript NR 134251.1 of APTR have an opposite function to all transcripts, providing a theoretical basis for the prevention and treatment of arsenic exposure.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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