Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles.
BACKGROUND: The incidence of intrahepatic cholangiocarcinoma (ICC) is increasing globally, and its prognosis has not improved substantially in recent years. Understanding the pathogenesis of ICC may provide a theoretical basis for its treatment. In this study, we investigated the effects and underly...
| Publicado en: | Clinical Medicine Insights: Oncology Vol. 17; pp. 1 - 11 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
Jan-Dec2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=175226903&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 175226903 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11795549 B3KT jtl: Clinical Medicine Insights: Oncology issn: 11795549 maglogo: Y pubinfo: dt: Jan-Dec2023 vid: 17 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 175226903 175226903 175226903 10.1177/11795549231181189 175226903 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Fucosyltransferase 5 Promotes the Proliferative and Migratory Properties of Intrahepatic Cholangiocarcinoma Cells via Regulating Protein Glycosylation Profiles. aug: au: Jingheng Guo Qian Cheng Yongjian Li Lingyu Tian Delin Ma Zhao Li Jie Gao Jiye Zhu affil: Department of Hepatobiliary Surgery, Peking University People's Hospital, Beijing, China sug: subj: Transferases Metabolism Proteins Metabolism Glycosylation Cell Proliferation Cholangiocarcinoma Cell Movement Human Immunohistochemistry Mass Spectrometry Reverse Transcriptase Polymerase Chain Reaction Cell Culture Techniques Cell Viability Blotting, Western Descriptive Statistics T-Tests Data Analysis Software Gene Expression Funding Source ab: BACKGROUND: The incidence of intrahepatic cholangiocarcinoma (ICC) is increasing globally, and its prognosis has not improved substantially in recent years. Understanding the pathogenesis of ICC may provide a theoretical basis for its treatment. In this study, we investigated the effects and underlying mechanisms of fucosyltransferase 5 (FUT5) on the malignant progression of ICC. METHODS: FUT5 expression in ICC samples and adjacent nontumor tissues was compared using quantitative real-time polymerase chain reaction and immunohistochemical assays. We performed cell counting kit-8, colony formation, and migration assays to determine whether FUT5 influenced the proliferation and mobility of ICC cells. Finally, mass spectrometry was performed to identify the glycoproteins regulated by FUT5. RESULTS: FUT5 mRNA was significantly upregulated in most ICC samples compared with corresponding adjacent nontumor tissues. The ectopic expression of FUT5 promoted the proliferation and migration of ICC cells, whereas FUT5 knockdown significantly suppressed these cellular properties. Mechanistically, we demonstrated that FUT5 is essential for the synthesis and glycosylation of several proteins, including versican, β3 integrin, and cystatin 7, which may serve key roles in the precancer effects of FUT5. CONCLUSIONS: FUT5 is upregulated in ICC and promotes ICC development by promoting glycosylation of several proteins. Therefore, FUT5 may serve as a therapeutic target for the treatment of ICC. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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