Dicer Suppresses Hepatocellular Carcinoma via Interleukin-8 Pathway.
BACKGROUND: Elevated level of interleukin-8 (IL-8) promotes hepatocellular carcinoma (HCC) development and contributes to poor prognosis. Previously, we have proved that Dicer inhibits HCC progression. In this study, we evaluated the potential interaction between IL-8 and Dicer as well as their infl...
| Publicado en: | Clinical Medicine Insights: Oncology Vol. 17; pp. 1 - 8 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
Jan-Dec2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=175226904&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 175226904 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11795549 B3KT jtl: Clinical Medicine Insights: Oncology issn: 11795549 maglogo: Y pubinfo: dt: Jan-Dec2023 vid: 17 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 175226904 175226904 175226904 10.1177/11795549231161212 175226904 ppf: 1 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Dicer Suppresses Hepatocellular Carcinoma via Interleukin-8 Pathway. aug: au: Xin Han Jianhua Wu Ziyue Sha Ruixue Lai Jianfei Shi Lili Mi Fei Yin Zhanjun Guo affil: Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, PR China sug: subj: Esterases Interleukins Blood Carcinoma, Hepatocellular Drug Therapy Carcinoma, Hepatocellular Prognosis Drug Interactions Evaluation Animal Studies Mice Funding Source Gene Expression Wound Healing Physiology Flow Cytometry Descriptive Statistics Immunoassay Cell Proliferation ab: BACKGROUND: Elevated level of interleukin-8 (IL-8) promotes hepatocellular carcinoma (HCC) development and contributes to poor prognosis. Previously, we have proved that Dicer inhibits HCC progression. In this study, we evaluated the potential interaction between IL-8 and Dicer as well as their influence on HCC. METHODS: Hepatocellular carcinoma cells of SMMC-7721 were divided into 2 groups for subsequent analysis: pCMV-Dicer group for Dicer-overexpressing lentivirus transfected cells (pCMV-Dicer cells) and pCMV-NC group for empty lentivirus transfected cells (pCMV-NC cells). Cell Counting kit-8 (CCK8), wound healing, and transwell were used to evaluate the inhibitory effect of Dicer overexpression on proliferation, migration, and invasion of HCC cells. The level of IL-8 was measured by flow cytometry bead-based immunoassays. Male nude BALB/c mice injected with pCMV-Dicer or pCMV-NC cell suspensions was used for transplant of HCC tumor. RESULTS: We found that the secretion of IL-8 was reduced in the medium of pCMV-Dicer cells (P = .027). Recombinant human IL-8 (rhIL-8) reversed the inhibitory effect of Dicer on proliferation (P < .01), migration (P = .003), and invasion (P = .001), whereas IL-8 inhibitor of reparixin enhanced inhibitory effect of Dicer on proliferation (P < .05), migration (P = .008), and invasion (P = .000). Lenvatinib downregulated the IL-8 level of HCC cells (P = .000) as well as promote Dicer-induced inhibition for HCC cells referring to proliferation (P < .05), migration (P = .000), and invasion (P = .000). Animal experiments also demonstrated that Dicer cooperated with lenvatinib to inhibit the growth of HCC tumors (P < .05). CONCLUSIONS: Dicer cooperated with lenvatinib to inhibit HCC growth via downregulating IL-8, and Dicer displayed its potential capability to enhance the anti-tumor effect of lenvatinib. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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