Occurrence of Escherichia coli O157:H7 infection and its effects on haematological parameters among patients in Central Hospital, Benin City, Nigeria.

Objectives: Escherichia coli O157:H7 has emerged as an important food-borne pathogen of considerable public health concern. This study was conducted against the background of the paucity of data on its prevalence in secondary healthcare facilities in Benin City, Nigeria. Methods: Stool and blood spe...

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Publicado en:New Zealand Journal of Medical Laboratory Science Vol. 77; no. 3; pp. 109 - 113
Autores principales: Elemuwa, Christopher O., Isibor, Joe O., Nyoho J. Inyang, Elemuwa, Uchenna Geraldine, Omoregie, Richard, Ayanlere, Mary
Formato: research tables/charts Journal Article
Publicado: New Zealand Institute of Medical Laboratory Science Nov2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Nov2023
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        atl: Occurrence of Escherichia coli O157:H7 infection and its effects on haematological parameters among patients in Central Hospital, Benin City, Nigeria.
      aug:
        au:
          Elemuwa, Christopher O.
          Isibor, Joe O.
          Nyoho J. Inyang
          Elemuwa, Uchenna Geraldine
          Omoregie, Richard
          Ayanlere, Mary
        affil: Research/Director, National Primary Healthcare Development Agency, Garki, Abuja, Nigeria
      sug:
        subj:
          Food Contamination Adverse Effects
          Escherichia Coli Infections Epidemiology
          Hematologic Tests Evaluation
          Public Health
          Human
          Nigeria
          Male
          Female
          Infant
          Child, Preschool
          Child
          Adolescence
          Adult
          Middle Age
          Aged
          Aged, 80 and Over
          Descriptive Statistics
          Chi Square Test
          Odds Ratio
          Data Analysis Software
          T-Tests
          Feces Analysis
          Blood Analysis
          Edetic Acid
          Prevalence
          Recovery
          Infant: 1-23 months
          Child, Preschool: 2-5 years
          Child: 6-12 years
          Adolescent: 13-18 years
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Aged: 65+ years
          Aged, 80 & over
          Male
          Female
      ab: Objectives: Escherichia coli O157:H7 has emerged as an important food-borne pathogen of considerable public health concern. This study was conducted against the background of the paucity of data on its prevalence in secondary healthcare facilities in Benin City, Nigeria. Methods: Stool and blood specimens were collected from 420 patients with various gastrointestinal complaints, accessing care at Central Hospital, Benin City, Nigeria. The stool specimens were cultured on MacConkey agar to recover Escherichia coli. The recovered Escherichia coli isolates were further subjected to sorbitol fermentation and the sorbitol non-fermenting isolates were further subjected to Escherichia coli O157:H7 latex agglutination serology. The blood specimens were used to determine the full blood count of the patients. Results: A total of 107 (25.48%) Escherichia coli were recovered from the 420 stool specimens. The gender of the patients did not significantly affect the distribution of the Escherichia coli isolates (p=0.9114). Patients within the age group of 11 - 20 years had significantly (p<0.0001) higher rates of Escherichia coli recovery and recovery of Escherichia coli was significantly associated with diarrheagenic stool specimens (OR= 2.376; 95%CI= 1.079, 5.232; p= 0.0489). A total of 7(6.54%) of the 107 Escherichia coli isolates were sorbitol non-fermenting and 5 (4.67%) of these were confirmed to be Escherichia coli O157:H7 serologically. The presence of Escherichia coli O157:H7 did not significantly alter the haematological parameters. Conclusion: The prevalence of Escherichia coli O157:H7 in this study was 1.19%, and its presence did not cause any changes in haematological parameters. Measures to prevent the spread of this food-borne pathogen are advocated.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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