The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis.
Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patient...
| Publicado en: | Nefrología Vol. 44; no. 2; pp. 194 - 204 |
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| Autores principales: | , , , , , , , |
| Formato: | Artículo |
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Revista Nefrologia
Mar/Apr2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=175923252&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 175923252 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 02116995 50C3 jtl: Nefrología issn: 02116995 maglogo: N pubinfo: dt: Mar/Apr2024 vid: 44 iid: 2 pid: 39358 pub: Revista Nefrologia artinfo: ui: 175923252 10.1016/j.nefro.2023.08.008 ppf: 194 ppct: 10 formats: fmt: @attributes: type: P size: 2MB tig: atl: The urogenital microbiome in chronic kidney disease patients on peritoneal dialysis. aug: au: Araujo, Ricardo Merino-Ribas, Ana Pereira, Luciano Campos, Joana Silva, Nádia Alencastre, Inês Soares Pestana, Manuel Sampaio-Maia, Benedita affil: Nephrology & Infectious Diseases R&D Group, i3S-Instituto de Investigac¸ão e Inovac¸ão em Saúde, INEB-Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal. Nephrology Department, Hospital Universitari de Girona Doctor Josep Trueta, Girona, Spain. Universitat Autònoma de Barcelona, Barcelona, Spain. Nephrology Department, Centro Hospitalar Universitário de São João, Porto, Portugal. Faculdade de Medicina Dentária, Universidade do Porto, Porto, Portugal. su: Chronic kidney failure Chronically ill Urinary tract infections Peritoneal dialysis Hemodialysis patients Peritoneum diseases Gut microbiota Porto (Portugal) sug: subj: Porto (Portugal) Chronic kidney failure Chronically ill Urinary tract infections Peritoneal dialysis Hemodialysis patients Peritoneum diseases Gut microbiota keyword: Chronic kidney diseas Diálisis peritoneal Enfermedad renal crónica Gut microbiome Human microbiome Infección del tracto urinario Microbioma humano Microbioma intestinal Microbioma urogenital Urinary tract infection Urobioma Urobiome Urogenital microbiome Diálisis peritoneal Enfermedad renal crónica Infección del tracto urinario Microbioma humano Microbioma intestinal Microbioma urogenital Urobioma ab: Introduction and objectives: Diabetes, dyslipidemia, older age, gender, urinary tract infections, and recent antibiotic intake have been associated with a decrease in the urobiome richness and other fluctuations in this microbiome. Gut and blood microbiome have been reported to be altered in patients with chronic kidney disease (CKD), and specifically in peritoneal dialysis (PD) patients. Still, there are currently no studies describing the urogenital microbiome in CKD-PD patients. In this study we characterized the urobiome profile in 46 PD patients and analyzed its clinical and inflammatory parameters. Materials and methods: Mid-stream urine, fecal and blood samples were collected from 46 patients undergoing PD at Centro Hospitalar Universitário de São João (CHUSJ) in Porto, Portugal. Exclusion criteria were age under 18 years old, inability to give informed consent, history of infection in the last three months, and antibiotic intake in the last three months. The microbiome communities were analyzed by amplification and sequencing of the V3-V4 region of the bacterial 16S rRNA gene. Correlations with the patients’ clinical data and inflammatory profile were performed. Results: CKD-PD patients presented a unique urobiome profile dominated by Bacillota, Actinomycetota and Pseudomonadota and characterized by a lower Shannon diversity than fecal and blood microbiome. The taxonomic profiles of urogenital samples were organized in multiple subtypes dominated by populations of Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, being similar to other non-PD-CKD patients. Gender, sCD14, residual diuresis and history of peritonitis were significantly associated to variations in the urobiome. Although not reaching statistical significance, diabetes and the time on PD also showed association with particular taxonomic groups. Depletion of Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus or Dermabacter populations correlated with CKD-PD patients with history of diabetes, history of peritonitis and altered levels of sCD14. Conclusions: Our results highlight urogenital microbiome as a potential partner and/or marker in the overall health state of CKD-PD patients. Introducción y objetivos: Diabetes, dislipemia, edad avanzada, género, infecciones del tracto urinario y toma reciente de antibióticos, entre otras, se han asociado a una disminución en la riqueza del urobioma y a otras fluctuaciones de dicho microbioma.Recientemente, se han descrito alteraciones en losmicrobiomas intestinal y en sangreen pacientes con enfermedad renal crónica (ERC) y, específicamente, en pacientes en diálisis peritoneal (DP).A pesar de ello, aún no existen estudios que describan el microbioma urogenital en pacientes en DP. En el presente trabajo, caracterizamos el urobioma en 46 pacientes en DP. Pacientes y métodos: Se recogieron muestras de orina (micción espontánea), heces y sangre de 46 pacientes en DP del Centro HospitalarUniversitário de São João en Oporto, Portugal. Los criterios de exclusión fueron edad menor a 18 anos, ˜ incapacidad para entenderel consentimiento informado, e historia de infección y toma de antibióticos en los últimos 3 meses. Las comunidades microbiológicas fueron analizadas por amplificación y secuenciación de las regiones V3-V4 del 16S rRNA bacteriano. Se realizaron correlaciones con los datos clínicos y el perfil inflamatorio de los pacientes. Resultados: Los pacientes en DP presentaron un urobioma único dominado por Bacillota, Actinomycetota yPseudomonadota, y caracterizado por una menor diversidad de Shannon que los microbiomas en sangre e intestinal. Los perfiles taxonómicos de las muestras urogenitales se organizaron en múltiples subtipos dominados por poblaciones de Lactobacillus, Staphylococcus, Streptococcus, Gardnerella, Prevotella, Escherichia-Shigella, siendo similar al descrito para otros pacientes con ERC no en DP.Género, factor sCD14, diuresis residual yantecedentes de peritonitis se asociaron de forma significativa a cambios en el urobioma. Diabetes y tiempo en DP también se asociaron con grupos taxonómicos específicos, aunque no de forma estadísticamente significativa. La deplección de Gardnerella, Staphylococcus, Corynebacterium, Lactobacillus o Dermabacterse correlacionó con antecedentes de diabetes, historia de peritonitis y niveles alterados de sCD14. Conclusiones: Nuestros resultados subrayan la importancia del microbioma urogenital como potencial biomarcadordel estado de salud de nuestros pacientes en DP. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Nefrología is the property of Revista Nefrologia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Nefrología holder: Revista Nefrologia dt: @attributes: year: 2024 holdings: @attributes: islocal: N |
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