Heterozygosity alters Msh5 binding to meiotic chromosomes in the baker's yeast.
Meiotic crossovers are initiated from programmed DNA double-strand breaks. The Msh4–Msh5 heterodimer is an evolutionarily conserved mismatch repair–related protein complex that promotes meiotic crossovers by stabilizing strand invasion intermediates and joint molecule structures such as Holliday jun...
| Publicado en: | Genetics Vol. 226; no. 3; pp. 1 - 19 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Mar2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=176103837&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 176103837 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Mar2024 vid: 226 iid: 3 pid: 622 pub: Oxford University Press / USA artinfo: ui: 176103837 176103837 176103837 10.1093/genetics/iyad214 176103837 ppf: 1 ppct: 18 formats: tig: atl: Heterozygosity alters Msh5 binding to meiotic chromosomes in the baker's yeast. aug: au: Dash, Suman Joshi, Sameer Pankajam, Ajith V Shinohara, Akira Nishant, Koodali T affil: School of Biology, Indian Institute of Science Education and Research Thiruvananthapuram , Trivandrum 695551 , India sug: subj: DNA-Binding Proteins Cell Nucleus Division Saccharomyces Analysis Heterozygote Chromosomes DNA Analysis Genome Sequence Analysis Polymorphism, Single Nucleotide In Vitro Studies DNA Repair DNA Damage Genes Blotting, Western Bioinformatics T-Tests Post Hoc Analysis Wilcoxon Rank Sum Test Linear Regression Pearson's Correlation Coefficient Funding Source ab: Meiotic crossovers are initiated from programmed DNA double-strand breaks. The Msh4–Msh5 heterodimer is an evolutionarily conserved mismatch repair–related protein complex that promotes meiotic crossovers by stabilizing strand invasion intermediates and joint molecule structures such as Holliday junctions. In vivo studies using homozygous strains of the baker's yeast Saccharomyces cerevisiae (SK1) show that the Msh4–Msh5 complex associates with double-strand break hotspots, chromosome axes, and centromeres. Many organisms have heterozygous genomes that can affect the stability of strand invasion intermediates through heteroduplex rejection of mismatch-containing sequences. To examine Msh4–Msh5 function in a heterozygous context, we performed chromatin immunoprecipitation and sequencing (ChIP-seq) analysis in a rapidly sporulating hybrid S. cerevisiae strain (S288c-sp/YJM789, containing sporulation-enhancing QTLs from SK1), using SNP information to distinguish reads from homologous chromosomes. Overall, Msh5 localization in this hybrid strain was similar to that determined in the homozygous strain (SK1). However, relative Msh5 levels were reduced in regions of high heterozygosity, suggesting that high mismatch densities reduce levels of recombination intermediates to which Msh4–Msh5 binds. Msh5 peaks were also wider in the hybrid background compared to the homozygous strain (SK1). We determined regions containing heteroduplex DNA by detecting chimeric sequence reads with SNPs from both parents. Msh5 -bound double-strand break hotspots overlap with regions that have chimeric DNA, consistent with Msh5 binding to heteroduplex-containing recombination intermediates. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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