Effects of a single phosphate-enriched test meal on inflammasome activity and postprandial inflammatory markers in healthy subjects.
Purpose: The consumption of highly processed food is often associated with a high intake of inorganic phosphate. Hyperphosphatemia is accompanied by an inflammatory status in patients with chronic kidney disease. However, the immune response to high phosphorus intake in healthy individuals is largel...
| Publicado en: | European Journal of Nutrition Vol. 63; no. 3; pp. 797 - 808 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Apr2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=176120917&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 176120917 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Apr2024 vid: 63 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 176120917 174593627 176120917 176120917 10.1007/s00394-023-03306-6 176120917 ppf: 797 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Effects of a single phosphate-enriched test meal on inflammasome activity and postprandial inflammatory markers in healthy subjects. aug: au: Nier, Anika Ulrich, Christof Volk, Christin Wolffgang, Marie-Charlotte Brandsch, Corinna Wensch-Dorendorf, Monika Girndt, Matthias Stangl, Gabriele I. affil: https://ror.org/05gqaka33 Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle, Germany sug: subj: Meals Phosphates Inflammation Postprandial Period Biological Markers Funding Source Human Descriptive Statistics Data Analysis Software T-Tests Wilcoxon Rank Sum Test Male Female Young Adult Adult Post Hoc Analysis Phosphorus Administration and Dosage Gene Expression Monocytes Nutrition Oxidative Stress Adult: 19-44 years Male Female ab: Purpose: The consumption of highly processed food is often associated with a high intake of inorganic phosphate. Hyperphosphatemia is accompanied by an inflammatory status in patients with chronic kidney disease. However, the immune response to high phosphorus intake in healthy individuals is largely unknown. Therefore, the aim of the present study was to evaluate the effect of a single phosphate-enriched meal on inflammasome activity and plasma levels of inflammatory markers. Methods: The analysis included 28 participants who received a single dose of either 700 mg phosphorus or a placebo with a test meal. At baseline, 4 and 8 h post-meal, plasma interleukin (IL)-6, IL-1β, IL-10, c-reactive protein (CRP), soluble IL-6 receptor (sIL-6R) and glycoprotein 130 (sgp130) levels were determined. At baseline and 4 h post-meal, peripheral blood mononuclear cells were isolated to assess inflammasome activity. Subsequently, the effect of phosphate with or without glucose on IL-6 and IL-1β gene expression and secretion in U937 monocytes was examined. Results: While both groups showed a marked postprandial increase in IL-6 plasma levels, neither plasma levels of IL-6, IL-1β, CRP, IL-10, sIL-6R, and sgp130 nor inflammasome activity were affected by phosphate compared to placebo. In U937 cells, there was also no effect of phosphate on IL-6 expression, but the addition of glucose increased it. Phosphate, however, reduced the IL-1β secretion of these cells. Conclusion: Postprandial inflammatory markers were not affected by dietary phosphate. However, IL-6 plasma levels were markedly increased post-meal, which appears to be a metabolic rather than a pro-inflammatory phenomenon. Trial registration number: ClinicalTrials.gov, NCT03771924, date of registration: 11th December 2018, retrospectively registered. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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