Bao Yuan decoction alleviates fatigue by restraining inflammation and oxidative stress via the AMPK/CRY2/PER1 signaling pathway.
Baoyuan Decoction (BYD) was initially recorded in the classic of "Bo Ai Xin Jian" in the Ming dynasty. It is traditionally used for treating weakness and cowardice, and deficiency of vital energy. In researches related to anti-fatigue effects, the reciprocal regulation of AMPK and circadian clocks l...
| Publicado en: | Journal of Ethnopharmacology Vol. 328 |
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| Autores principales: | , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Jun2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=176541239&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 176541239 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Jun2024 vid: 328 pid: 1004 pub: Elsevier B.V. artinfo: ui: 176541239 176541239 176541239 10.1016/j.jep.2024.118058 176541239 ppct: 1 formats: tig: atl: Bao Yuan decoction alleviates fatigue by restraining inflammation and oxidative stress via the AMPK/CRY2/PER1 signaling pathway. aug: au: Zheng, Yuan Ren, Xueyang Qi, Xiaodan Song, Ruolan Zhao, Chongjun Ma, Jiamu Li, Xianxian Deng, Qingyue He, Yingyu Kong, Lingmei Qian, Liyan Zhang, Feng Li, Mingxia Sun, Mengyu Liu, Wei Liu, Haibin She, Gaimei affil: School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China sug: subj: Drugs, Chinese Herbal Pharmacodynamics Drugs, Chinese Herbal Pharmacokinetics Network Pharmacology Gene Expression Profiling Fatigue Drug Therapy Inflammation Drug Therapy Oxidative Stress Drug Effects Signal Transduction Drug Effects Biological Markers Metabolism Models, Biological Animal Studies Fish Reverse Transcriptase Polymerase Chain Reaction Antioxidants Pharmacodynamics AMP-Activated Protein Kinases Drug Effects Chromatography, High Pressure Liquid Liquid Chromatography-Mass Spectrometry Biological Markers Drug Effects Interleukins Gene Expression Drug Effects RNA, Messenger Drug Effects Drugs, Chinese Herbal Analysis ab: Baoyuan Decoction (BYD) was initially recorded in the classic of "Bo Ai Xin Jian" in the Ming dynasty. It is traditionally used for treating weakness and cowardice, and deficiency of vital energy. In researches related to anti-fatigue effects, the reciprocal regulation of AMPK and circadian clocks likely plays an important role in anti-fatigue mechanism, while it has not yet been revealed. Therefore, we elucidated the anti-fatigue mechanism of BYD through AMPK/CRY2/PER1 pathway. To investigate the effect and mechanism of BYD in reducing fatigue, using pharmacodynamics, network pharmacology and transcriptomics through the AMPK/CRY2/PER1 signaling pathway. Firstly, the chemical constituents of BYD were qualitatively identified by UHPLC-Q-Exactive Orbitrap/MS, establishing a comprehensive strategy with an in-house library, Xcalibur software and Pubchem combined. Secondly, a Na 2 SO 3 -induced fatigue model and 2,2′-Azobis (2-methylpropionamidine) dihydrochloride (AAPH)-induced oxidative stress model were developed to evaluate the anti-fatigue and anti-oxidant activities of BYD using AB zebrafish. The anti-inflammatory activity of BYD was evaluated using CuSO 4 -induced and tail cutting-induced Tg (lyz: dsRed) transgenic zebrafish inflammation models. Then, target screening was performed by Swiss ADME, GeneCards, OMIM and DrugBank databases, the network was constructed using Cytoscape 3.9.0. Transcriptome and network pharmacology technology were used to investigate the related signaling pathways and potential mechanisms after treatment with BYD, which were verified by real-time quantitative PCR (RT-qPCR). In total, 114 compounds from the water extract of BYD were identified as major compounds. Na₂SO₃-induced fatigue model and AAPH-induced oxidative stress model indicated that BYD has significant anti-fatigue and antioxidant effects. Meanwhile, BYD showed significant anti-inflammatory effects on CuSO 4 -induced and tail cutting-induced zebrafish inflammation models. The KEGG result of network pharmacology showed that the anti-fatigue function of BYD was mainly effected through AMPK signaling pathway. Besides, transcriptome analysis indicated that the circadian rhythm, AMPK and IL-17 signaling pathways were recommended as the main pathways related to the anti-fatigue effect of BYD. The RT-qPCR results showed that compared with a model control group, the treatment of BYD significantly elevated the expression mRNA of AMPK, CRY2 and PER1. Herein, we identified 114 chemical constituents of BYD, performed zebrafish activity validation, while demonstrated that BYD can relieve fatigue by AMPK/CRY2/PER1 signaling pathway through network pharmacology and transcriptome. [Display omitted] • One hundred and fourteen chemical components of BYD were identified by UHPLC-Q-Exactive Orbitrap/MS technology. • BYD significantly inhibited Na 2 SO 3 -induced fatigue and AAPH-induced oxidation in zebrafish. • BYD showed significant anti-inflammatory effects on CuSO 4 -induced and tail cutting-induced zebrafish inflammation models. • We obtained AMPK/CRY2/PER1 signaling pathway through integrated analysis of network pharmacology and transcriptomic. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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