Sensitive, Label-Free DNA Diagnostics Based on Near-Field Microwave Imaging.
The article presents a study of the sensitive, label-free DNA diagnostics based on near-field microwave imaging (NFMI). Label-free diagnostics remain attractive as they simplify sample preparation, decrease assay costs, and eliminate potential artifacts from label instability or perturbation of assa...
| Publicado en: | Journal of the American Chemical Society Vol. 127; no. 27; pp. 9666 - 9668 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
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American Chemical Society
7/13/2005
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=17658273&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 17658273 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 7/13/2005 vid: 127 iid: 27 pid: 997 pub: American Chemical Society artinfo: ui: 17658273 10.1021/ja051760i ppf: 9666 ppct: 2 formats: tig: atl: Sensitive, Label-Free DNA Diagnostics Based on Near-Field Microwave Imaging. aug: au: Friedman, Barry Gaspar, Mariafrancis A. Kalachikov, Sergey Kiejin Lee Levicky, Rastislav Gang Shen Hyunjun Yoo affil: Department of Physics, Sam Houston State University, Huntsville, Texas 77341. Department of Chemical Engineering, Columbia University, New York, New York 10027. Columbia Genome Center, Columbia University, New York, New York 10032. Department of Physics and interdisciplinary Program of Integrated Biotechnology, Sogang University, Seoul 121-742, Korea. su: Diagnostic imaging Imaging systems DNA Nucleic acids Thermodynamics Physical & theoretical chemistry sug: subj: Diagnostic imaging Imaging systems DNA Nucleic acids Thermodynamics Physical & theoretical chemistry ab: The article presents a study of the sensitive, label-free DNA diagnostics based on near-field microwave imaging (NFMI). Label-free diagnostics remain attractive as they simplify sample preparation, decrease assay costs, and eliminate potential artifacts from label instability or perturbation of assay thermodynamics. NFMI functions by sensing changes in electrostatic coupling between a 'probe' and the sample. The remarkable sensitivity recommends NFMI for applications that require detection of trace surface species. The principal advantage of NFMI over other label-free detection methods is outstanding sensitivity, thus removing a central obstacle to label-free diagnostics. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2005 holdings: @attributes: islocal: N |
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