Construction of circular RNA‐based competing endogenous RNA network in colon cancer.

Circular RNAs (CircRNAs) are now under discussion as novel promising biomarkers for patients with various tumors. Our study aimed to identify circRNAs related to the progression of colon cancer and to further investigate their roles in the development of colon cancer base on The Cancer Genome Atlas...

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Publicado en:Precision Medical Sciences Vol. 13; no. 1; pp. 52 - 63
Autores principales: Gu, Zhengyu, Wu, Weiyi, Lu, Jinnan, Shen, Jun
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Mar2024
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Mar2024
      vid: 13
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1002/prm2.12127
        176586021
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        atl: Construction of circular RNA‐based competing endogenous RNA network in colon cancer.
      aug:
        au:
          Gu, Zhengyu
          Wu, Weiyi
          Lu, Jinnan
          Shen, Jun
        affil: Department of Gastroenterology, Nanjing Red Cross Hospital, Nanjing, China
      sug:
        subj:
          RNA, Circular
          Colonic Neoplasms
          Tumor Markers, Biological
          Genome
          Cancer Patients
          Human
          Male
          Female
          Middle Age
          Aged
          Disease Progression
          Genes
          Correlational Studies
          Gene Expression Profiling
          MicroRNA
          Data Analysis Software
          Age Factors
          Sex Factors
          Neoplasm Staging
          Survival
          Sensitivity and Specificity
          Cell Line, Tumor
          Database Management Software
          Quantitative Studies
          T-Tests
          Comparative Studies
          Mann-Whitney U Test
          Kaplan-Meier Estimator
          Log-Rank Test
          Univariate Statistics
          Multivariate Analysis
          Cox Proportional Hazards Model
          Middle Aged: 45-64 years
          Aged: 65+ years
          Male
          Female
      ab: Circular RNAs (CircRNAs) are now under discussion as novel promising biomarkers for patients with various tumors. Our study aimed to identify circRNAs related to the progression of colon cancer and to further investigate their roles in the development of colon cancer base on The Cancer Genome Atlas database. The competing endogenous RNA (ceRNA) network was constructed based on 4 circRNAs, 14 miRNAs, and 229 mRNAs. Meanwhile, three hub genes were identified via the protein protein interaction networks (PPI) network. It should be noted that only RUNX1 targeted by circ‐0084615 and mir‐330‐3p was confirmed as a survival‐related gene. Further analyses indicated the possible function of RUNX1 in colon progression, such as angiogenesis, epithelial–mesenchymal transition, hedgehog signaling and so on. Further investigations were conducted into the correlation between RUNX1 expression profiles and immune cells. Finally, we validated the expression of the circ‐0084615/mir‐330‐3p/RUNX1 axis in clinical specimens. In conclusion, we constructed ceRNA network and revealed that the circ‐0084615/mir‐330‐3p/RUNX1 axis serves as a critical role in colon cancer.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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