Evaluation of Multidrug Resistance and TEM and SHV BroadSpectrum Beta-Lactamase Genes in Escherichia coli Obtained from Patients with Urinary Tract Infections in Alborz Province, Iran.
Background & Objective: Investigating multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections is very useful to improve the treatment of infection and prevent the failure of treatment of urinary tract...
| Publicado en: | Journal of Advances in Medical & Biomedical Research Vol. 32; no. 150; pp. 79 - 87 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Zanjan University of Medical Sciences & Health Services
Jan/Feb2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=177406284&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 177406284 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26766264 N5MJ jtl: Journal of Advances in Medical & Biomedical Research issn: 26766264 maglogo: N pubinfo: dt: Jan/Feb2024 vid: 32 iid: 150 pid: 65276 pub: Zanjan University of Medical Sciences & Health Services artinfo: ui: 177406284 177406284 177406284 177406284 ppf: 79 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Evaluation of Multidrug Resistance and TEM and SHV BroadSpectrum Beta-Lactamase Genes in Escherichia coli Obtained from Patients with Urinary Tract Infections in Alborz Province, Iran. aug: au: Soltan Dallal, Mohammad Mehdi Mirbagheri, Seyedeh Zohre Vahedi, Saeed Mohammadi, Mohammad Reza Agha Mirzaei, Hedroosha Molla affil: Dept of Pathobiology, School of Health, Tehran University of Medical Sciences, Tehran, Iran. sug: subj: Urinary Tract Infections Physiopathology Drug Resistance, Multiple Evaluation beta-Lactamases Metabolism Genes Evaluation Escherichia Coli Analysis Human Iran Treatment Failure Urine Specimen Collection Culture Media Culture Techniques Escherichia Coli Metabolism Polymerase Chain Reaction Microbial Culture and Sensitivity Tests Descriptive Statistics Imipenem Therapeutic Use Carbapenems Therapeutic Use Descriptive Research Academic Medical Centers Genome Evaluation DNA Analysis Electrophoresis Spectrophotometers Staining and Labeling Sensitivity and Specificity Ceftazidime Therapeutic Use Cefotaxime Therapeutic Use ab: Background & Objective: Investigating multidrug resistance and TEM and SHV broad-spectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections is very useful to improve the treatment of infection and prevent the failure of treatment of urinary tract infections.The aim of this study was to investigate multidrug resistance and TEM and SHV broadspectrum beta-lactamase genes in Escherichia coli bacteria isolated from patients with urinary tract infections. Materials & Methods: In this study, 188 strains of Escherichia coli, which cause urinary tract infections in Alborz province, were studied. Urine samples were cultured on EMB and Blood Agar media. Differential tests were performed for final identification. ESBL-producing strains were identified, PCR was performed to survey the abundance of ESBL-producing genes. Results: Based on the results of the disk diffusion and Double-disk synergy testS, 82 (43.6%) strains were determined as the final producer of ESBL. out of these isolates, the frequency of SHV, TEM, and CTX genes measured 64.3%, 55.9%, and 21.4%, respectively. These results showed that 12 (14.28%) of Escherichia coli isolated have all genes, 26 (30.95%) had 2 genes and 36 (42.85%) had one gene. Conclusion: According to the results, it was found that imipenem with the lowest resistance is the best drug in the treatment, and carbapenems are the best drug for treating diseases caused by Escherichia coli. The results of the current study may be useful in replacing ESBL enzyme resistance screening with more modern sensitivity measurement methods such as MIC and Etest. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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