Donepezil promotes skin flap survival through activation of the HIF‐1α/VEGF signalling pathway.
Flaps are mainly used to repair wounds in the clinical setting but can sometimes experience ischaemic necrosis postoperatively. This study investigated whether donepezil, an acetylcholinesterase inhibitor, can enhance the survival rate of flaps. We randomly allocated 36 rats into control, low‐dose (...
| Publicado en: | Wound Repair & Regeneration Vol. 32; no. 4; pp. 500 - 511 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178355120&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178355120 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul2024 vid: 32 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 178355120 178355120 178355120 10.1111/wrr.13176 178355120 ppf: 500 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Donepezil promotes skin flap survival through activation of the HIF‐1α/VEGF signalling pathway. aug: au: Lin, Hang Wang, Kaitao Yang, Jialong Wang, An Deng, Jiapeng Lin, Dingsheng affil: Department of Hand and Plastic Surgery, The Second School of Medicine, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou Zhejiang, , China sug: subj: Donepezil Pharmacodynamics Surgical Flaps Skin Transplantation Vascular Endothelial Growth Factors Drug Effects Signal Transduction Drug Effects Health Promotion Methods Graft Survival Drug Effects Proteins Drug Effects Animal Studies Mice Random Assignment Postoperative Period Tissue Viability Evaluation Molecular Biology Apoptosis Drug Effects Autophagy Drug Effects Neovascularization, Physiologic Drug Effects Interleukins Descriptive Statistics Biochemical Phenomena Funding Source ab: Flaps are mainly used to repair wounds in the clinical setting but can sometimes experience ischaemic necrosis postoperatively. This study investigated whether donepezil, an acetylcholinesterase inhibitor, can enhance the survival rate of flaps. We randomly allocated 36 rats into control, low‐dose (3 mg/kg/day), and high‐dose (5 mg/kg/day) groups. On Postoperative day 7, we assessed flap viability and calculated the mean area of viable flap. After euthanizing the rats, we employed immunological and molecular biology techniques to examine the changes in flap tissue vascularization, apoptosis, autophagy, and inflammation. Donepezil enhanced the expression of hypoxia‐inducible factor and vascular endothelial growth factor to facilitate angiogenesis. In addition, it elevated the expression of LC3B, p62, and beclin to stimulate autophagy. Furthermore, it increased the expression of Bcl‐2 while reducing the expression of Bax, thus inhibiting apoptosis. Finally, it had anti‐inflammatory effects by reducing the levels of IL‐1β, IL‐6, and TNF‐α. The results suggest that donepezil can enhance the viability of randomly generated skin flaps by upregulating HIF‐1α/VEGF signalling pathway, facilitating vascularization, inducing autophagy, suppressing cell apoptosis, and mitigating inflammation within the flap tissue. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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