Low‐temperature plasma jet suppresses bacterial colonisation and affects wound healing through reactive species.
An argon‐based low‐temperature plasma jet (LTPJ) was used to treat chronically infected wounds in Staphylococcus aureus‐laden mice. Based on physicochemical property analysis and in vitro antibacterial experiments, the effects of plasma parameters on the reactive nitrogen and oxygen species (RNOS) c...
| Publicado en: | Wound Repair & Regeneration Vol. 32; no. 4; pp. 407 - 419 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178355122&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178355122 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul2024 vid: 32 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 178355122 176494186 178355122 178355122 10.1111/wrr.13178 178355122 ppf: 407 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Low‐temperature plasma jet suppresses bacterial colonisation and affects wound healing through reactive species. aug: au: Ge, Yang Wang, Jun Gu, DongHua Cao, Wei Feng, Yongtong Wu, Yanfan Liu, Han Xu, Zhengping Zhang, Zhe Xie, Jinsong Geng, Shuang Cong, Junrui Liu, Yi affil: The CAS Key Laboratory of Bio‐Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou Jiangsu,, China sug: subj: Cold Temperature Bacterial Colonization Wound Healing Sterilization and Disinfection Methods Reactive Nitrogen Species Reactive Oxygen Species Animal Studies Funding Source Models, Biological Mice In Vitro Studies Antiinfective Agents Therapeutic Use In Vivo Studies MMPI Colony Count, Microbial Collagen Inflammation Prevention and Control Transforming Growth Factor beta Treatment Outcomes Wounds, Chronic Therapy ab: An argon‐based low‐temperature plasma jet (LTPJ) was used to treat chronically infected wounds in Staphylococcus aureus‐laden mice. Based on physicochemical property analysis and in vitro antibacterial experiments, the effects of plasma parameters on the reactive nitrogen and oxygen species (RNOS) content and antibacterial capacity were determined, and the optimal treatment parameters were determined to be 4 standard litre per minute and 35 W. Additionally, the plasma‐treated activation solution had a bactericidal effect. Although RNOS are related to the antimicrobial effect of plasma, excess RNOS may be detrimental to wound remodelling. In vivo studies demonstrated that medium‐dose LTPJ promoted MMP‐9 expression and inhibited bacterial growth during the early stages of healing. Moreover, LTPJ increased collagen deposition, reduced inflammation, and restored blood vessel density and TGF‐β levels to normal in the later stages of wound healing. Therefore, when treating chronically infected wounds with LTPJ, selecting the medium dose of plasma is more advantageous for wound recovery. Overall, our study demonstrated that low‐temperature plasma jets may be a potential tool for the treatment of chronically infected wounds. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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