Phellodendron chinense C.K.Schneid: An in vitro study on its anti-Helicobacter pylori effect.
Phellodendron chinense C.K.Schneid(P. chinense Schneid) is known in TCM as Huang Bo , is traditionally used to support gastrointestinal function and alleviate stomach-related ailments, including gastric ulcer bleeding and symptoms of gastroesophageal reflux disease. Helicobacter pylori (H. pylori) i...
| Publicado en: | Journal of Ethnopharmacology Vol. 333 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Oct2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178399657&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178399657 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Oct2024 vid: 333 pid: 1004 pub: Elsevier B.V. artinfo: ui: 178399657 178399657 178399657 10.1016/j.jep.2024.118396 178399657 ppct: 1 formats: tig: atl: Phellodendron chinense C.K.Schneid: An in vitro study on its anti-Helicobacter pylori effect. aug: au: Chen, Meiyun Wu, Ziyao Zou, Yuanjing Peng, Chang Hao, Yajie Zhu, Zhixiang Shi, Xiaoyan Su, Bingmei Ou, Ling Lai, Yuqian Jia, Junwei Xun, Mingjin Li, Hui Zhu, Weixing Feng, Zhong Yao, Meicun affil: School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Shenzhen, 518107, China sug: subj: Medicine, Chinese Traditional Evaluation Plant Extracts Pharmacodynamics Plants, Medicinal Pharmacodynamics Helicobacter Pylori Drug Effects Gastroesophageal Reflux Drug Therapy Gastrointestinal Hemorrhage Drug Therapy Antiinfective Agents Phytochemicals Analysis In Vitro Studies Reverse Transcriptase Polymerase Chain Reaction Staining and Labeling Fluorescent Antibody Technique Gene Expression Profiling Virulence ab: Phellodendron chinense C.K.Schneid(P. chinense Schneid) is known in TCM as Huang Bo , is traditionally used to support gastrointestinal function and alleviate stomach-related ailments, including gastric ulcer bleeding and symptoms of gastroesophageal reflux disease. Helicobacter pylori (H. pylori) is classified by the WHO as a Group 1 carcinogen. However, the specific activity and mechanism of action of P. chinense Schneid against H. pylori infection remain unclear. It has been noted that Huangjiu processing may alter the bitter and cold properties of P. chinense Schneid , but its effect on antimicrobial activity requires further investigation. Additionally, it remains uncertain whether berberine is the sole antimicrobial active component of P. chinense Schneid. This study aims to elucidate the anti- H. pylori infection activity of P. chinense Schneid , along with its mechanism of action and key antimicrobial active components. Phytochemical analysis was carried out by UPLC-MS/MS. HPLC was employed to quantify the berberine content of the extracts. Antimicrobial activity was assessed using the micro broth dilution method. Morphology was observed using SEM. The impact on urease activity was analyzed through in vitro urease enzyme kinetics. RT-qPCR was employed to detect the expression of virulence genes, including adhesin, flagellum, urease, and cytotoxin-related genes. The adhesion effect was evaluated by immunofluorescence staining and agar culture. P. chinense Schneid exhibited strong antimicrobial activity against both antibiotic-sensitive and resistant H. pylori strains, with MIC ranging from 40 to 160 μg/mL. Combination with amoxicillin, metronidazole, levofloxacin, and clarithromycin did not result in antagonistic effects. P. chinense Schneid induced alterations in bacterial morphology and structure, downregulated the expression of various virulence genes, and inhibited urease enzyme activity. In co-infection systems, P. chinense Schneid significantly attenuated H. pylori adhesion and urease relative content, thereby mitigating cellular damage caused by infection. Huangjiu processing enhanced the anti- H. pylori activity of P. chinense Schneid. Besides berberine, P. chinense Schneid contained seven other components with anti- H. pylori activity, with palmatine exhibiting the strongest activity, followed by jatrorrhizine. This study sheds light on the potential therapeutic mechanisms of P. chinense Schneid against H. pylori infection, demonstrating its capacity to disrupt bacterial structure, inhibit urease activity, suppress virulence gene transcription, inhibit adhesion, and protect host cells. The anti -H. pylori activity of P. chinense Schneid was potentiated by Huangjiu processing, and additional components beyond berberine were identified as possessing strong anti- H. pylori activity. Notably, jatrorrhizine, a core component of P. chinense Schneid , exhibited significant anti- H. pylori activity, marking a groundbreaking discovery. [Display omitted] • Phytochemical constituents of HB were analyzed. • HB show anti- H p infection activity, revealing its ability to disrupt bacterial structure, inhibit urease activity, decrease the transcription of virulence genes, inhibit adhesion, and protect host cells. • The anti- H p activity of HB was enhanced after Huangjiu processing, with MIC of 40 μg/ml. • A groundbreaking discovery was the strong anti- H p activity of jatrorrhizine, one of the core components of HB. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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