Reagent storage and delivery on integrated microfluidic chips for point-of-care diagnostics.
Microfluidic-based point-of-care diagnostics offer several unique advantages over existing bioanalytical solutions, such as automation, miniaturisation, and integration of sensors to rapidly detect on-site specific biomarkers. It is important to highlight that a microfluidic POC system needs to perf...
| Publicado en: | Biomedical Microdevices Vol. 26; no. 3; pp. 1 - 34 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178445314&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178445314 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Sep2024 vid: 26 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 178445314 10.1007/s10544-024-00709-y 178445314 ppf: 1 ppct: 33 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Reagent storage and delivery on integrated microfluidic chips for point-of-care diagnostics. aug: au: Rasekh, Manoochehr Harrison, Sam Schobesberger, Silvia Ertl, Peter Balachandran, Wamadeva affil: https://ror.org/00dn4t376 College of Engineering, Design and Physical Sciences, Brunel University London, UB8 3PH, Uxbridge, UK sug: ab: Microfluidic-based point-of-care diagnostics offer several unique advantages over existing bioanalytical solutions, such as automation, miniaturisation, and integration of sensors to rapidly detect on-site specific biomarkers. It is important to highlight that a microfluidic POC system needs to perform a number of steps, including sample preparation, nucleic acid extraction, amplification, and detection. Each of these stages involves mixing and elution to go from sample to result. To address these complex sample preparation procedures, a vast number of different approaches have been developed to solve the problem of reagent storage and delivery. However, to date, no universal method has been proposed that can be applied as a working solution for all cases. Herein, both current self-contained (stored within the chip) and off-chip (stored in a separate device and brought together at the point of use) are reviewed, and their merits and limitations are discussed. This review focuses on reagent storage devices that could be integrated with microfluidic devices, discussing further issues or merits of these storage solutions in two different sections: direct on-chip storage and external storage with their application devices. Furthermore, the different microvalves and micropumps are considered to provide guidelines for designing appropriate integrated microfluidic point-of-care devices. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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