Statistical Experimental Designs for cLTB-Syn Vaccine Production Using Daucus carota Cell Suspension Cultures.
The carrot-made LTB-Syn antigen (cLTB-Syn) is a vaccine candidate against synucleinopathies based on carrot cells expressing the target antigen LTB and syn epitopes. Therefore, the development of an efficient production process is required with media culture optimization to increase the production y...
| Publicado en: | Planta Medica Vol. 90; no. 10; pp. 744 - 757 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Georg Thieme Verlag Stuttgart
2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178834116&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178834116 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00320943 NH9 jtl: Planta Medica issn: 00320943 maglogo: N pubinfo: dt: 2024 vid: 90 iid: 10 pid: 4536 pub: Georg Thieme Verlag Stuttgart artinfo: ui: 178834116 178834116 178834116 10.1055/a-2307-0400 178834116 ppf: 744 ppct: 13 formats: tig: atl: Statistical Experimental Designs for cLTB-Syn Vaccine Production Using Daucus carota Cell Suspension Cultures. aug: au: Carreño-Campos, Christian Villegas, Elba Villarreal, María Luisa Morales-Aguilar, Mónica Govea-Alonso, Dania Romero-Maldonado, Andrea Jimenez-Capdeville, María E. Rosales-Mendoza, Sergio Ortiz-Caltempa, Anabel affil: Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, México sug: subj: Synucleinopathies Carrot Cell Line Metabolism Urea Callus Tissue Culture Techniques Methods Culture Media Vaccines Immunology Parkinson Disease Prevention and Control Immunotherapy Animal Studies Mice Ecosystem Excitatory Amino Acid Agents Post Hoc Analysis Descriptive Statistics T-Tests Analysis of Variance Models, Statistical Gene Expression Proteins Analysis Cell Viability Genomics Immunization Plants, Medicinal ab: The carrot-made LTB-Syn antigen (cLTB-Syn) is a vaccine candidate against synucleinopathies based on carrot cells expressing the target antigen LTB and syn epitopes. Therefore, the development of an efficient production process is required with media culture optimization to increase the production yields as the main goal. In this study, the effect of two nitrogen sources (urea and glutamate) on callus cultures producing cLTB-Syn was studied, observing that the addition of 17 mM urea to MS medium favored the biomass yield. To optimize the MS media composition, the influence of seven medium components on biomass and cLTB-Syn production was first evaluated by a Plackett–Burman design (PBD). Then, three factors were further analyzed using a central composite design (CCD) and response surface methodology (RSM). The results showed a 1.2-fold improvement in biomass, and a 4.5-fold improvement in cLTB-Syn production was achieved at the shake-flask scale. At the bioreactor scale, there was a 1.5-fold increase in biomass and a 2.8-fold increase in cLTB-Syn yield compared with the standard MS medium. Moreover, the cLTB-Syn vaccine induced humoral responses in BALB/c mice subjected to either oral or subcutaneous immunization. Therefore, cLTB-Syn is a promising vaccine candidate that will aid in developing immunotherapeutic strategies to combat PD and other neurodegenerative diseases without the need for cold storage, making it a financially viable option for massive immunization. pubtype: Periodical doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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