Trichilia catigua against Helicobacter pylori: An in vitro, molecular and in silico approach.
Helicobacter pylori is a bacterium that is present in the stomach of about 50% of the global population and is associated with several gastric disorders, including cancer. Natural products with antimicrobial activity have been tested against H. pylori , among them Trichilia catigua (catuaba), which...
| Publicado en: | Fitoterapia Vol. 177 |
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| Autores principales: | , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Sep2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=178885795&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 178885795 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0367326X JF4 jtl: Fitoterapia issn: 0367326X maglogo: N pubinfo: dt: Sep2024 vid: 177 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 178885795 178885795 178885795 10.1016/j.fitote.2024.106101 178885795 ppct: 1 formats: tig: atl: Trichilia catigua against Helicobacter pylori: An in vitro, molecular and in silico approach. aug: au: Ritter, Mariane Roberta Oliveira, Marcelo Tempesta de Ardisson, Juliana Santa Gonçalves, Rita de Cássia Ribeiro Kitagawa, Rodrigo Rezende Lima, Diego de Souza Seixas, Flavio Augusto Vicente Nakamura, Celso Vataru Mello, João Carlos Palazzo de affil: Laboratory of Pharmaceutical Biology, Palafito, Department of Pharmacy, Universidade Estadual de Maringá, Maringá, Brazil sug: subj: Helicobacter Infections Drug Therapy Helicobacter Infections Prevention and Control Plant Extracts Pharmacodynamics Plant Extracts Therapeutic Use Plant Bark In Vitro Studies Plant Extracts Analysis Virulence Polymerase Chain Reaction Antiinfective Agents Microscopy, Electron, Scanning Molecular Docking Simulation Gene Expression Clarithromycin Fatty Acids Drug Effects Descriptive Statistics ab: Helicobacter pylori is a bacterium that is present in the stomach of about 50% of the global population and is associated with several gastric disorders, including cancer. Natural products with antimicrobial activity have been tested against H. pylori , among them Trichilia catigua (catuaba), which is widely distributed in Brazil. This study aimed to evaluate extracts of T. catigua bark against H. pylori via determination of the minimum inhibitory and bactericidal concentrations (MIC and MBC); evaluation of virulence factors by real-time PCR, synergism with standard antimicrobials and morphology by scanning electron microscopy and simulations of the mechanism of action by molecular docking. The ethyl acetate fraction provided the best results, with an MIC 50 of 250 μg/mL and a 42.34% reduction in urease activity, along with reduced expression of the CagA and VacA genes, which encode for the main virulence factors. This fraction presented synergistic activity with clarithromycin, reducing the MIC of the drug by four-fold. Docking simulations suggested that the extracts inhibit fatty acid synthesis by the FAS-II system, causing damage to the cell membrane. Therefore, T. catigua extracts have potential as an adjuvant to treatment and are promising for the development of new anti- H. pylori drugs. [Display omitted] • Helicobacter pylori is a bacterium associated with the development of stomach cancer. • Trichilia catigua extracts showed a synergistic effect with clarithromycin and amoxicillin. • The extracts caused a reduction in the gene expression of the main virulence factors. • Molecular Docking analysis suggested interference with the H. pylori cell membrane. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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