Evaluating the Anti-Migratory Effect of Fucoxanthin in Human Cisplatin-Resistant Ovarian Cancer Cells.
Background & Objective: Ovarian cancer ranks among the most prevalent gynecologic cancers in women with the majority of patients experiencing a recurrent type and developing treatment resistance. Resistance to chemotherapy drugs has been observed to trigger epithelial-mesenchymal transition (EMT), w...
| Publicado en: | Journal of Advances in Medical & Biomedical Research Vol. 32; no. 151; pp. 135 - 144 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Zanjan University of Medical Sciences & Health Services
Mar/Apr2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=179303473&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 179303473 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26766264 N5MJ jtl: Journal of Advances in Medical & Biomedical Research issn: 26766264 maglogo: N pubinfo: dt: Mar/Apr2024 vid: 32 iid: 151 pid: 65276 pub: Zanjan University of Medical Sciences & Health Services artinfo: ui: 179303473 179303473 179303473 10.30699/jambr.32.151.135 179303473 ppf: 135 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Evaluating the Anti-Migratory Effect of Fucoxanthin in Human Cisplatin-Resistant Ovarian Cancer Cells. aug: au: Sani, Fatemeh Valinezhad Ghofrani, Shiva Kamalian, Safa Gharaee, Melika Ehtesham Mosaffa, Fatemeh affil: Department of Laboratory Sciences, School of Paramedical and Rehabilitation Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. sug: subj: Xanthophylls Therapeutic Use Cisplatin Therapeutic Use Antineoplastic Agents Therapeutic Use Drug Resistance, Neoplasm Evaluation Ovarian Neoplasms Drug Therapy Cell Line, Tumor Drug Efficacy Human Funding Source Female Adult Epithelial-Mesenchymal Transition Chemotherapy, Cancer Cell Migration Assays Wound Healing Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Cell Viability Spectrophotometry Descriptive Statistics One-Way Analysis of Variance Gene Expression Adult: 19-44 years Female ab: Background & Objective: Ovarian cancer ranks among the most prevalent gynecologic cancers in women with the majority of patients experiencing a recurrent type and developing treatment resistance. Resistance to chemotherapy drugs has been observed to trigger epithelial-mesenchymal transition (EMT), which leads to the acquisition of metastatic properties. Thus, targeting the molecular mechanism of EMT may lead to novel interventions against metastatic disease. In this research, the suppressive impacts of fucoxanthin, as a natural compound, were examined on cell migration and expression of key EMT-related markers in ovarian cancer cells which were sensitive and resistant to cisplatin. Materials & Methods: To determine the non-toxic concentrations of fucoxanthin for migration assay, the MTT assay was conducted. To assess the anti-migratory capacity of fucoxanthin in ovarian cancer cells which were sensitive and resistant to cisplatin, a wound-healing migration test was done in the presence of non-toxic concentrations of fucoxanthin. RT-qPCR was utilized to examine the impact of fucoxanthin on the mRNA expression of E-cadherin, vimentin and α- SMA (as EMT-related markers) in ovarian cancer cells. Results: Fucoxanthin at concentrations of (1 and 2.5 μM) downregulated the expression of α–SMA in cisplatin-resistant ovarian cancer cells and inhibited migration in ovarian cancer cell lines. Conclusion: Fucoxanthin exhibited remarkable efficacy in inhibiting migration in in ovarian cancer cells which were sensitive and resistant to cisplatin. However, more research is required to ascertain the clinical advantages linked to utilizing fucoxanthin in the treatment of cisplatin-resistant ovarian cancers. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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