Short and long-term 2100 MHz radiofrequency radiation causes endoplasmic reticulum stress in rat testis.
Long-term radiofrequency radiation (RFR) exposure, which adversely affects organisms, deteriorates testicular functions. Misfolding or unfolding protein accumulation in the endoplasmic reticulum (ER) initiates an intracellular reaction known as ER stress (ERS), which activates the unfolded protein r...
| Publicado en: | Histochemistry & Cell Biology Vol. 162; no. 4; pp. 311 - 322 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Oct2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=179357789&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 179357789 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Oct2024 vid: 162 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 179357789 178378838 10.1007/s00418-024-02308-7 179357789 ppf: 311 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Short and long-term 2100 MHz radiofrequency radiation causes endoplasmic reticulum stress in rat testis. aug: au: Kirimlioglu, Esma Oflamaz, Asli Okan Hidisoglu, Enis Ozen, Sukru Yargicoglu, Piraye Demir, Necdet affil: https://ror.org/01m59r132 Departments of Histology and Embryology, Faculty of Engineering, Akdeniz University, Antalya, Turkey sug: ab: Long-term radiofrequency radiation (RFR) exposure, which adversely affects organisms, deteriorates testicular functions. Misfolding or unfolding protein accumulation in the endoplasmic reticulum (ER) initiates an intracellular reaction known as ER stress (ERS), which activates the unfolded protein response (UPR) for proteostasis. Since both RFR exposure and ERS can cause male infertility, we hypothesized that RFR exposure causes ERS to adversely affect testicular functions in rats. To investigate role of ERS in mediating RFR effects on rat testis, we established five experimental groups in male rats: control, short-term 2100-megahertz (MHz) RFR (1-week), short-term sham (sham/1-week), long-term 2100-MHz RFR (10-week), and long-term sham (sham/10-week). ERS markers Grp78 and phosphorylated PERK (p-Perk) levels and ERS-related apoptosis markers Chop and caspase 12 were investigated by immunohistochemistry, immunoblotting, and quantitative real-time polymerase chain reaction (qPCR). Long-term RFR exposure increased Grp78, p-Perk, and Chop levels, while short-term RFR exposure elevated Chop and caspase 12 levels. Chop expression was not observed in spermatogonia and primary spermatocytes, which may protect spermatogonia and primary spermatocytes against RFR-induced ERS-mediated apoptosis, thereby allowing transmission of genetic material to next generations. While short and long-term RFR exposures trigger ERS and ERS-related apoptotic pathways, further functional analyses are needed to elucidate whether this RFR-induced apoptosis has long-term male infertility effects. Article Highlights: Long-term radiofrequency radiation in testis triggers endoplasmic reticulum stress. Short-term radiofrequency radiation causes apoptosis in testis. Spermatogonia and spermatocytes are not affected by short-term radiofrequency radiation. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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