Genomic Insights of a Methicillin‐Resistant Biofilm‐Producing Staphylococcus aureus Strain Isolated From Food Handlers.
Methicillin‐resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of...
| Publicado en: | BioMed Research International Vol. 2024; pp. 1 - 15 |
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| Autores principales: | , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
7/20/2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=179674137&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 179674137 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/20/2024 vid: 2024 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 179674137 179674137 179674137 10.1155/2024/5516117 179674137 ppf: 1 ppct: 14 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Genomic Insights of a Methicillin‐Resistant Biofilm‐Producing Staphylococcus aureus Strain Isolated From Food Handlers. aug: au: Ballah, Fatimah Muhammad Hoque, M. Nazmul Islam, Md. Saiful Faisal, Golam Mahbub Rahman, Al-Muksit Mohammad Taufiquer Khatun, Mst. Minara Rahman, Marzia Hassan, Jayedul Rahman, Md. Tanvir Wan, Shibiao affil: Department of Microbiology and Hygiene,, Faculty of Veterinary Science,, Bangladesh Agricultural University,, Mymensingh, 2202,, Bangladesh, bau.edu.bd sug: subj: Genomics Methicillin-Resistant Staphylococcus Aureus Biofilms Drug Effects Food Handling Drug Resistance, Microbial Sequence Analysis Human Male Female Funding Source Bangladesh Antiinfective Agents Bioinformatics Virulence Phenotype Genome Amino Acids Metabolism Carbohydrates Proteins Metabolism Vitamins Metabolism Minerals Metabolism Lipids Metabolism Genetic Variation Descriptive Statistics Male Female ab: Methicillin‐resistant Staphylococcus aureus (MRSA) is an important zoonotic pathogen associated with a wide range of infections in humans and animals. Thus, the emergence of MRSA clones poses an important threat to human and animal health. This study is aimed at elucidating the genomics insights of a strong biofilm‐producing and multidrug‐resistant (MDR) S. aureus MTR_BAU_H1 strain through whole‐genome sequencing (WGS). The S. aureus MTR_BAU_H1 strain was isolated from food handlers' hand swabs in Bangladesh and phenotypically assessed for antimicrobial susceptibility and biofilm production assays. The isolate was further undergone to high throughput WGS and analysed using different bioinformatics tools to elucidate the genetic diversity, molecular epidemiology, sequence type (ST), antimicrobial resistance, and virulence gene distribution. Phenotypic analyses revealed that the S. aureus MTR_BAU_H1 strain is a strong biofilm‐former and carries both antimicrobial resistance (e.g., methicillin resistance; mecA, beta‐lactam resistance; blaZ and tetracycline resistance; tetC) and virulence (e.g., sea, tsst, and PVL) genes. The genome of the S. aureus MTR_BAU_H1 belonged to ST1930 that possessed three plasmid replicons (e.g., rep16, rep7c, and rep19), seven prophages, and two clustered regularly interspaced short palindromic repeat (CRISPR) arrays of varying sizes. Phylogenetic analysis showed a close evolutionary relationship between the MTR_BAU_H1 genome and other MRSA clones of diverse hosts and demographics. The MTR_BAU_H1 genome harbours 42 antimicrobial resistance genes (ARGs), 128 virulence genes, and 273 SEED subsystems coding for the metabolism of amino acids, carbohydrates, proteins, cofactors, vitamins, minerals, and lipids. This is the first‐ever WGS‐based study of a strong biofilm‐producing and MDR S. aureus strain isolated from human hand swabs in Bangladesh that unveils new information on the resistomes (ARGs and correlated mechanisms) and virulence potentials that might be linked to staphylococcal pathogenesis in both humans and animals. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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