Evaluation of Antibiotic Sensitivity, Biofilm Formation Ability, and Prevalence of Extended-Spectrum Beta-Lactamase (ESBLs) in Clinically Isolates Escherichia coli and Klebsiella pneumoniae.
Background & Objective: Extended-spectrum beta-lactamases (ESBLs) are enzymes in bacteria that resist many antibiotics. Detection of ESBLs production is important as it's a marker of colonization and potential transfer to other patients. We studied the antibiotic susceptibility, biofilm formation ca...
| Publicado en: | Journal of Advances in Medical & Biomedical Research Vol. 32; no. 152; pp. 219 - 226 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Zanjan University of Medical Sciences & Health Services
May/Jun2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=180180884&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 180180884 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26766264 N5MJ jtl: Journal of Advances in Medical & Biomedical Research issn: 26766264 maglogo: N pubinfo: dt: May/Jun2024 vid: 32 iid: 152 pid: 65276 pub: Zanjan University of Medical Sciences & Health Services artinfo: ui: 180180884 180180884 180180884 10.30699/jambs.32.152.219 180180884 ppf: 219 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Evaluation of Antibiotic Sensitivity, Biofilm Formation Ability, and Prevalence of Extended-Spectrum Beta-Lactamase (ESBLs) in Clinically Isolates Escherichia coli and Klebsiella pneumoniae. aug: au: Mirzaei, Bahman Shirmohammadpour, Mina Safarzadeh, Hanieh Jafari, Sajjad affil: Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran. sug: subj: Antibiotics Drug Resistance, Microbial Biofilms Escherichia Coli Klebsiella beta-Lactamases Human Phenotype Funding Source Ampicillin Tetracycline Health Policy Microbial Culture and Sensitivity Tests Polymerase Chain Reaction ab: Background & Objective: Extended-spectrum beta-lactamases (ESBLs) are enzymes in bacteria that resist many antibiotics. Detection of ESBLs production is important as it's a marker of colonization and potential transfer to other patients. We studied the antibiotic susceptibility, biofilm formation capacity, and prevalence of ESBLs of opportunistic bacteria including K. pneumoniae and E. coli. The isolates capable of biofilm formation were analyzed among 100 E. coli and 104 K. pneumoniae isolates. Materials & Methods: This process involved collecting and identifying bacterial samples, testing antibiotic susceptibility, detecting ESBLs phenotypes, and multidrug-resistance (MDR) isolates, assessing biofilm formation capability, and evaluating results through statistical analysis. Results: The susceptibility tests for discs were performed following the guidelines outlined by the Clinical Laboratory Standards Institute in 2023 (CLSI). K. pneumoniae exhibits inherent resistance to ampicillin, while 80 (80%) strains of E. coli have been reported to be resistant to ampicillin. Additionally, 50 K. pneumoniae isolates and 41 E. coli isolates were found capable of forming a biofilm. Seven of E. coli (17.07%), and seven of K. pneumoniae (14%) isolates could form a mighty biofilm. It was observed that the strongest resistance in the isolates that formed strong biofilm was related to tetracycline with 5 (7.2%) resistance in K. pneumonia and 7 (7%) resistance in E. coli. Furthermore, 47 (47%) of E. coli, and 21 (20.2%) of K. pneumoniae isolates were classified as ESBLs producers, and 52 (50%) K. pneumoniae and 72 (72%) E. coli isolates were classified as MDR. Conclusion: Considering the role of biofilm in the transfer of genes, appropriate health policies, and the correct administration of effective antibiotics can help in prevention. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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