Mechanism of Treadmill Exercise Combined with Rich Environmental Stimulation to Improve Depression in Post-stroke Depression Model Rats.

Background: Post-stroke depression (PSD) is a common complication, occurring in approximately one-third of these patients. The neurological symptoms of PSD affect patients' daily life and subsequent recovery. Analyzing the pathogenesis of post-stroke depression from a psychological perspective, it w...

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Published in:Actas Espanolas de Psiquiatria Vol. 52; no. 5; pp. 693 - 705
Main Authors: Lina Lu, Wenting Xu, Lin Feng, Xiuying Teng, Weigu Ban, Yuman Xue, Xueliang Xing, Wen Zeng, Baolong Li
Format: Article
Published: Maria Lopez-Ibor 2024
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      dt: 2024
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      pub: Maria Lopez-Ibor
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        atl: Mechanism of Treadmill Exercise Combined with Rich Environmental Stimulation to Improve Depression in Post-stroke Depression Model Rats.
      aug:
        au:
          Lina Lu
          Wenting Xu
          Lin Feng
          Xiuying Teng
          Weigu Ban
          Yuman Xue
          Xueliang Xing
          Wen Zeng
          Baolong Li
        affil:
          Rehabilitation Department 2, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China 2Spine and Scoliosis Clinic, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China 3Rehabilitation Department 4, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China 4Postgraduate College, Heilongjiang University of Chinese Medicine, 150040 Harbin, Heilongjiang, ChinaRehabilitation Department 2, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China
          Spine and Scoliosis Clinic, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China
          Rehabilitation Department 4, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China
          Postgraduate College, Heilongjiang University of Chinese Medicine, 150040 Harbin, Heilongjiang, China
          Rehabilitation Medicine Basic Teaching and Research Office, The Second Clinical Medical College of Heilongjiang University of Chinese Medicine, 150000 Harbin, Heilongjiang, China
      su:
        Liquid chromatography-mass spectrometry
        Laboratory rats
        Mental depression
        Treadmill exercise
        Exercise physiology
      sug:
        subj:
          Liquid chromatography-mass spectrometry
          Laboratory rats
          Mental depression
          Treadmill exercise
          Exercise physiology
      keyword:
        kynurenine pathway
        post-stroke depression
        rich environmental stimulation
        treadmill exercise
      ab: Background: Post-stroke depression (PSD) is a common complication, occurring in approximately one-third of these patients. The neurological symptoms of PSD affect patients' daily life and subsequent recovery. Analyzing the pathogenesis of post-stroke depression from a psychological perspective, it was found that PSD patients often feel despair and anxiety, and it is crucial to explore nonpharmacological ways to improve post-stroke depressive symptoms. A combination of exercise and rich environmental stimulation (RES) has been found effective in improving post-stroke depressive symptoms. Therefore, this study aimed to explore the effects of exercise and rich environmental stimulation on PSD in rats and their potential underlying mechanisms and to provide a theoretical basis for managing PSD. Methods: The PSD rat model was constructed, and the depression-like behaviors of rats in each group were evaluated using the open field test (OFT), sucrose preference test (SPT), and forced swimming test (FST). Moreover, changes in the morphological behavior of rat hippocampus were observed using hematoxylin-eosin (HE) staining and Nissl staining. The expression levels of 5-hydroxytryptamine (5-HT) and norepinephrine (NE) in hippocampus tissues were assessed using enzyme linked immunosorbent assay (ELISA), and the levels of tryptophan-related proteins were determined employing western blot analysis. Additionally, a kynurenine-3-monooxygenase (KMO) inhibitor was administered to the combined stimulation group, and the levels of tryptophan (TRP), 5-HT, kynurenine (KYN), 3-hydroxy-kynurenine (3-HK), and quinolinic acid (QA) were evaluated using liquid chromatography mass spectrometry/mass spectrometry (LC-MS/MS). Results: Treadmill exercise combined with rich environmental stimulation significantly reduced the immobility time in the FST (p < 0.01), increased the exploratory behavior in the OFT (p < 0.05), and increased the sucrose water consumption in the SPT (p < 0.01), indicating that the depression-like behavior was improved. Treadmill exercise combined with rich environmental stimulation also improved the shape of the damaged hippocampus and increased the number of neurons in the hippocampus. Additionally, treadmill exercise combined with rich environmental stimulation significantly increased the levels of 5-HT and NE in hippocampus tissues (p < 0.01) and decreased KMO protein level (p < 0.01). In the KMO inhibitor group, the neural function was efficiently restored, the levels of 3-HK, QA, and KMO in the hippocampus were substantially reduced (p < 0.01), and the expression level of 5-HT was increased (p < 0.01). Conclusions: Exercise stimulation combined with enriched environmental stimuli alleviates post-stroke depression in rats, and the underlying mechanisms may be related to TRP/KYN/3-HK/QA excitotoxicity pathways and increased 5-hydroxytryptamine levels.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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      holder: Maria Lopez-Ibor
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          year: 2024
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