Inter- and Intra-observer Reproducibility of Thyroid Fine Needle Aspiration Cytology: An investigation of Bethesda 2023 Using Immunohistochemical BRAFV600E Antibody.
Background: The Bethesda System for Reporting Thyroid Cytology (TBSRTC) recommended for the interpretation of needle aspiration cytology of the thyroid, is the most widely used worldwide. Studies have shown that the disagreement between observers, especially in the Bethesda III and IV diagnostic cat...
| Publicado en: | Journal of Cytology Vol. 41; no. 4; pp. 221 - 229 |
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| Autores principales: | , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wolters Kluwer India Pvt Ltd
Oct-Dec2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=180829126&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 180829126 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09709371 58DU jtl: Journal of Cytology issn: 09709371 maglogo: N pubinfo: dt: Oct-Dec2024 vid: 41 iid: 4 pid: 16919 pub: Wolters Kluwer India Pvt Ltd artinfo: ui: 180829126 180829126 180829126 10.4103/joc.joc_26_24 180829126 ppf: 221 ppct: 8 formats: tig: atl: Inter- and Intra-observer Reproducibility of Thyroid Fine Needle Aspiration Cytology: An investigation of Bethesda 2023 Using Immunohistochemical BRAFV600E Antibody. aug: au: Bahattin, Erdogan Emine, Dündar Kısmet Çivi, Çetin Fatih, Yılmaz affil: Department of Pathology, Eskisehir City Hospital, Eskisehir, Turkey sug: subj: Thyroid Gland Pathology Thyroid Diseases Diagnosis Biopsy, Needle Cytology Methods Cytodiagnosis Reproducibility of Results Observer Bias Immunohistochemistry Human Aspiration Microbiology Thyroid Nodule Pathology Thyroid Neoplasms Diagnosis Diagnostic Errors Prevention and Control Molecular Diagnostic Techniques Antibodies, Monoclonal Descriptive Statistics kappa Statistic Sensitivity and Specificity Interrater Reliability ab: Background: The Bethesda System for Reporting Thyroid Cytology (TBSRTC) recommended for the interpretation of needle aspiration cytology of the thyroid, is the most widely used worldwide. Studies have shown that the disagreement between observers, especially in the Bethesda III and IV diagnostic categories, is not insignificant at 10%–40%. In the TBSRTC 2023 version, some definitions were removed and simplified, and molecular pathology was proposed as a complement to cytopathology. The current availability of molecular tests is limited because they can be performed in a few centers and are expensive. Therefore, our study investigated intra- and inter-observer agreement according to TBSRTC 2023 using only immunohistochemically BRAFV600E antibodies. Materials and Methods: The study included 173 cases with aspiration cytology evaluated between 2019 and 2022. The immunohistochemical procedure applied BRAFV600E (RM8) monoclonal antibody to cell block sections. All slides were assessed and categorized by three different observers. Data were interpreted using Cohen's kappa and Fleiss's kappa test in the Statistical Package for Social Sciences Windows 2021 program. Results: For the applied RM8 antibody, sensitivity was 64.71% and specificity was 87.27%. In terms of diagnostic categories, inter-observer agreement was good for Bethesda II (K = 0.606) and moderate for Bethesda III (K = 0.429), Bethesda IV (K = 0.523), Bethesda V (K = 0,464), and Bethesda VI (K = 0.544), respectively. Conclusion: In conclusion, the study reveals that the 2023 version of TBSRTC provides improvement, especially in the categories of uncertain diagnosis, but is still insufficient to improve cytological diagnostic accuracy, at which point molecular analyses become even more important. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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