The effect of anatomic location on porcine models of burn injury and wound healing.
Porcine models are frequently used for burn healing studies; however, factors including anatomic location and lack of standardised wound methods can impact the interpretation of wound data. The objectives of this study are to examine the influence of anatomical locations on the uniformity of burn cr...
| Publicado en: | Wound Repair & Regeneration Vol. 32; no. 5; pp. 675 - 686 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Sep2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=180986445&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 180986445 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Sep2024 vid: 32 iid: 5 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 180986445 177369486 180986445 180986445 10.1111/wrr.13190 180986445 ppf: 675 ppct: 11 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: The effect of anatomic location on porcine models of burn injury and wound healing. aug: au: Liu, Aiping Cheong, J. Z. Alex Hassan, Sameeha Wielgat, Matthew B. Meudt, Jennifer J. Townsend, Elizabeth Catherine Shanmuganayagam, Dhanansayan Kalan, Lindsay R. Gibson, Angela affil: Department of Surgery, University of Wisconsin‐Madison, Madison Wisconsin,, USA sug: subj: Burns Wounds and Injuries Wound Healing Models, Biological Animal Studies Funding Source Swine In Vivo Studies Lactate Dehydrogenase Staining and Labeling Burns Pathology Comparative Studies Surgical Wound Molecular Biology ab: Porcine models are frequently used for burn healing studies; however, factors including anatomic location and lack of standardised wound methods can impact the interpretation of wound data. The objectives of this study are to examine the influence of anatomical locations on the uniformity of burn creation and healing in porcine burn models. To optimise burn parameters on dorsal and ventral surfaces, ex vivo and in situ euthanized animals were first used to examine the location‐dependence of the burn depth and contact time relationship. The location‐dependent healing in vivo was then examined using burn and excisional wounds at dorsal, ventral, caudal and cranial locations. Lactate dehydrogenase (LDH) and H&E were used to assess burn depth and wound re‐epithelialization. We found that burn depth on the ventral skin was significantly deeper than that of the dorsal skin at identical thermal conditions. Compared with burns created ex vivo, burns created in situ immediately post‐mortem were significantly deeper in the ventral location. In live animals, 2 out of 12 burn wounds were fully re‐epithelialized after 14 days in contrast to complete re‐epithelialization of all excisional wounds. Among the burn wounds, those at the cranial‐dorsal site exhibited faster healing than at the caudal‐dorsal site. This study showed that anatomical location is an important consideration for the consistency of burn depth creation and healing. These data support symmetric localization of treatment and control for comparative assessment of burn healing in porcine models to prevent misinterpretation of results and increase the translatability of findings to humans. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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