Placenta‐derived mesenchymal stem cells promote diabetic wound healing via exosomal protein interaction networks.

There is a lack of effective treatment options for diabetic refractory wounds, which presents a critical clinical issue that needs to be addressed urgently. Our research has demonstrated that human placenta‐derived mesenchymal stem cells (plaMSCs) facilitate the migration and proliferation of HaCat...

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Publicado en:Wound Repair & Regeneration Vol. 32; no. 5; pp. 638 - 652
Autores principales: Peng, Cheng, Xu, Hongbo, Zhuang, Quan, Liu, Jinya, Ding, Yinhe, Tang, Qiyu, Wang, Zheng, Yao, Kai
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Sep2024
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Sep2024
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1111/wrr.13199
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        atl: Placenta‐derived mesenchymal stem cells promote diabetic wound healing via exosomal protein interaction networks.
      aug:
        au:
          Peng, Cheng
          Xu, Hongbo
          Zhuang, Quan
          Liu, Jinya
          Ding, Yinhe
          Tang, Qiyu
          Wang, Zheng
          Yao, Kai
        affil: Department of Burns and Plastic Surgery, The Third Xiangya Hospital of Central South University, Changsha, China
      sug:
        subj:
          Diabetes Mellitus Complications
          Diabetic Foot Therapy
          Wound Care Methods
          Placenta
          Mesenchymal Stem Cells Transplantation
          Wound Healing Evaluation
          Exosomes
          Proteins
          Signal Transduction
          Human
          Funding Source
          Cell Movement
          Keratinocytes
          Cell Line
          Proteomics
          Gene Expression
          Bioinformatics
          Extracellular Matrix
          Cell Physiology
          Cell Proliferation
          Neovascularization, Physiologic
      ab: There is a lack of effective treatment options for diabetic refractory wounds, which presents a critical clinical issue that needs to be addressed urgently. Our research has demonstrated that human placenta‐derived mesenchymal stem cells (plaMSCs) facilitate the migration and proliferation of HaCat cells, thereby enhancing diabetic wound healing primarily via the exosomes derived from plaMSCs (plaMSCs‐Ex). Using label‐free proteomics, plaMSCs and their exosomes were analysed for proteome taxonomic content in order to explore the underlying effective components mechanism of plaMSCs‐Ex in diabetic wound healing. Differentially expressed proteins enriched in plaMSCs‐Ex were identified and underwent bioinformatics analysis including GO annotation, KEGG pathway enrichment, gene set enrichment analysis (GSEA) and protein–protein interaction analysis (PPI). Results showed that the proteins enriched in plaMSCs‐Ex are significantly involved in extracellular matrix organisation, epithelium morphogenesis, cell growth, adhesion, proliferation and angiogenesis. PPI analysis filtered 2 wound healing‐related clusters characterised by hub proteins such as POSTN, FN1, SPARC, TIMP1, SERPINE1, LRP1 and multiple collagens. In brief, the exosomal proteins derived from plaMSCs reveal diverse functions of regeneration and tissue remodelling based on proteomics analysis and potentially play a role in diabetic wound healing.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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