Association of biofilm and microbial metrics with healing rate in older adults with chronic venous leg ulcers.
The presence of microbial biofilms in many human chronic wounds led to the hypothesis that biofilms delay healing of these wounds. We tested this hypothesis in a population of 117 older individuals with venous leg ulcers who were receiving standardised therapy, including frequent debridement. Debrid...
| Publicado en: | Wound Repair & Regeneration Vol. 32; no. 6; pp. 858 - 872 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Nov2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181057826&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181057826 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov2024 vid: 32 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 181057826 180324287 181057826 181057826 10.1111/wrr.13230 181057826 ppf: 858 ppct: 14 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Association of biofilm and microbial metrics with healing rate in older adults with chronic venous leg ulcers. aug: au: Stewart, Philip S. Kim, Junglyun James, Garth Yi, Fan Stechmiller, Joyce Weaver, Michael Kelly, Debra L. Fisher, Steve Schultz, Gregory Lyon, Debra affil: Center for Biofilm Engineering, Montana State University, Bozeman Montana,, USA sug: subj: Biofilms Wounds, Chronic Prevention and Control Venous Ulcer Psychosocial Factors Leg Ulcer Prevention and Control Wounds, Chronic Therapy Venous Ulcer Therapy Leg Ulcer Therapy Debridement Methods Hypothesis Wound Healing Venous Ulcer Microbiology Venous Ulcer Physiopathology Colony Count, Microbial Human Funding Source Aged Aged, 80 and Over Nonexperimental Studies Prospective Studies Culture Media Chronic Disease Ecosystem Wound Infection Microbiology Antiinfective Agents Therapeutic Use Descriptive Statistics Aged: 65+ years Aged, 80 & over ab: The presence of microbial biofilms in many human chronic wounds led to the hypothesis that biofilms delay healing of these wounds. We tested this hypothesis in a population of 117 older individuals with venous leg ulcers who were receiving standardised therapy, including frequent debridement. Debridement specimens were analysed for the amount of bacterial biomass by two independent methods: a microscopic approach that scored the relative size and number of bacterial aggregates, interpreted as a biofilm metric, and conventional enumeration by agar plating for viable bacteria. The plating protocol yielded three distinct values: the total viable bacterial count, bleach‐tolerant bacteria, and the log reduction in viable bacteria upon bleach treatment. Wound healing rates over an 8‐week observation period were calculated as the rate of decrease of the equivalent diameter of the wound. There was no statistically significant association between wound healing and the biofilm metric in any of the three analyses performed (p ≥0.15). In all three statistical tests, wound healing was associated with the log reduction caused by bleach treatment (p ≤0.004); wounds that harboured bacteria that were more bleach‐susceptible healed more slowly. A refinement of the model of chronic wound infection pathogenesis is proposed in which dormant bacteria constitute a persistent nidus and outgrowth of metabolically active cells impairs healing. This model constitutes a new hypothesis as metabolic activity was not directly measured in this investigation. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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