Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study.
Background: This study aimed to determine the alteration of endometrial expression levels of HOXA9/HOXA10/ HOXAII/HOXDIO genes and HOX transcript antisense intergenic RNA (HOTAIR) in mid-luteal phase endometrium in patients with hydrosalpinx before and after salpingectomy. Materials and Methods: In...
| Publicado en: | International Journal of Fertility & Sterility Vol. 18; no. 4; pp. 344 - 352 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Royan Institute
Oct-Dec2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181111714&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181111714 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 2008076X 92IA jtl: International Journal of Fertility & Sterility issn: 2008076X maglogo: N pubinfo: dt: Oct-Dec2024 vid: 18 iid: 4 pid: 57371 pub: Royan Institute artinfo: ui: 181111714 181111714 181111714 10.22074/IJFS.2024.2026081.1655 181111714 ppf: 344 ppct: 8 formats: tig: atl: Laparoscopic Salpingectomy Regulates The Expression of Endometrial Homeobox Genes (HOXA9, HOXA10, HOXA11, HOXD10) and HOX Transcript Antisense Intergenic RNA in Women with Communicating Hydrosalpinx: A Prospective Study. aug: au: Zafarani, Fatemeh Mehranjani, Malek Soleimani Ghaffari, Firouzeh Shahhoseini, Maryam Ali Shariatzadeh, Seyed Mohammad affil: Department of Biology, Faculty of Science, Arak University, Arak, Iran sug: subj: Surgery, Laparoscopic Salpingectomy Methods Gene Expression RNA Hydrosalpinx Surgery Endometrium Luteal Phase Human Female Adult Prospective Studies Iran Infertility Hysterosalpingography Endosonography Body Mass Index Menstrual Cycle Progesterone Blood Case Control Studies Biopsy DNA Polymerase Chain Reaction RNA, Messenger Descriptive Statistics Adult: 19-44 years Female ab: Background: This study aimed to determine the alteration of endometrial expression levels of HOXA9/HOXA10/ HOXAII/HOXDIO genes and HOX transcript antisense intergenic RNA (HOTAIR) in mid-luteal phase endometrium in patients with hydrosalpinx before and after salpingectomy. Materials and Methods: In this prospective study, 14 infertile women with unilateral hydrosalpinx who were scheduled for laparoscopic salpingectomy were evaluated. The presence of hydrosalpinx was confirmed by hyster-osalpingography or transvaginal 2D-ultrasonography. All patients had normal hormonal profiles, body mass index, and regular menstrual cycles identified by mid-luteal serum progesterone. Fourteen healthy fertile age-matched women with a successful pregnancy history were considered the control group. Mid-luteal-phase endometrial biopsies were performed at the time of surgery and during the mid-luteal phase of the fourth treatment cycle by Pipelle. After tissue collection, RNA extraction and cDNA synthesis were performed. Real-time polymerase chain reaction (PCR) technique was used for quantitative gene expression of HOXA9/HOXA10/HOXA11/HOXDIO and lnc HOTAIR. Results: The endometrial expression of HOXA9 (P<0.001), HOXA10 (P=0.001), HOXA11 (P=0.003), and HOXD10 (P=0.004) were significantly lower in the patients with hydrosalpinx compared to the controls. After salpingectomy, we observed a significant increase in the endometrial HOXA9 (P=0.006) and HOXA10 (P=0.023) mRNA expression levels compared to before salpingectomy samples. Similarly, a significant upregulation in endometrial HOXA11 (P= 0.013) and HOXA10 mRNA expression levels (P=0.012) were detected in postoperative samples compared to pre-operative tissue. Moreover, the lnc HOTAIR was significantly higher in the endometrium-induced hydrosalpinx fluid than in controls (P=0.020), which had a 2.89-fold decrease following salpingectomy (P=0.010). Conclusion: Elevated endometrial lncRNA HOTAIR may disrupt the expression of endometrial receptivity HOX genes in women with hydrosalpinx. However, our results failed to show a significant inverse correlation between HO-TAIR and HOX genes due to limited sample size. Further studies with larger sample sizes are required to investigate HOTAIR inverse co-expression with HOX genes in these subjects. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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