PET imaging of synaptic vesicle glycoprotein 2 subtype A for neurological recovery in ischemic stroke.

Purpose: [18F]SynVesT-1 is a novel radiopharmaceutical for assessing synaptic density in vivo. This study aims to investigate the potential of [18F]SynVesT-1 positron emission tomography (PET) in evaluating neurological recovery in the rat model of ischemic stroke, and to compare its performance wit...

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Published in:European Journal of Nuclear Medicine & Molecular Imaging Vol. 52; no. 1; pp. 158 - 171
Main Authors: Luo, Xiaoyun, Jin, Chentao, Chen, Hetian, Niu, Jiaqi, Yu, Congcong, Dou, Xiaofeng, Wang, Jing, Wen, Junjie, Zhang, Hong, Tian, Mei, Zhong, Yan
Format: Journal Article
Published: Springer Nature Dec2024
Online Access:View this record in EBSCOhost
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      dt: Dec2024
      vid: 52
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      pub: Springer Nature
      place: New York, New York
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        179291437
        10.1007/s00259-024-06904-6
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        atl: PET imaging of synaptic vesicle glycoprotein 2 subtype A for neurological recovery in ischemic stroke.
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          Luo, Xiaoyun
          Jin, Chentao
          Chen, Hetian
          Niu, Jiaqi
          Yu, Congcong
          Dou, Xiaofeng
          Wang, Jing
          Wen, Junjie
          Zhang, Hong
          Tian, Mei
          Zhong, Yan
        affil: https://ror.org/059cjpv64 Department of Nuclear Medicine and PET Center, The Second Affiliated Hospital of Zhejiang University School of Medicine, 88 Jiefang Road, 310009, Hangzhou, Zhejiang, China
      sug:
      ab: Purpose: [18F]SynVesT-1 is a novel radiopharmaceutical for assessing synaptic density in vivo. This study aims to investigate the potential of [18F]SynVesT-1 positron emission tomography (PET) in evaluating neurological recovery in the rat model of ischemic stroke, and to compare its performance with [18F]FDG PET. Methods: Sprague-Dawley rats were subjected to photothrombotic cerebral infarction, and safinamide was administered intraperitoneally from day 3 to day 14 post-stroke to alleviate neurological deficits. Cylinder test and forelimb placing test were performed to assess the neurological function. MRI, [18F]SynVesT-1 PET/CT and [18F]FDG PET/CT imaging were used to evaluate infarct volume, synaptic density, and cerebral glucose metabolism pre- and post-treatment. [18F]SynVesT-1 and [18F]FDG PET images were compared using Statistical Parametric Mapping (SPM) and region of interest (ROI)-based analysis. Post-mortem histological analysis was performed to validate PET images. Results: Safinamide treatment improved behavioral outcomes in stroke-damaged rats. Both [18F]SynVesT-1 and [18F]FDG PET detected stroke-induced injury, with the injured region being significantly larger in [18F]FDG PET than in [18F]SynVesT-1 PET. Compared with the saline group, radiotracer uptake in the injured area significantly increased in [18F]SynVesT-1 PET after safinamide treatment, whereas no notable change was observed in [18F]FDG PET. Additionally, [18F]SynVesT-1 PET imaging showed a better correlation with neurological function recovery than [18F]FDG PET. Post-mortem analysis revealed increased neuronal numbers, synaptic density, and synaptic neuroplasticity, as well as decreased glia activation in the stroke-injured area after treatment. Conclusion: [18F]SynVesT-1 PET effectively quantified spatiotemporal dynamics of synaptic density in the rat model of stroke, and showed different capabilities in detecting stroke injury and neurological recovery compared with [18F]FDG PET. The utilization of [18F]SynVesT-1 PET holds promise as a potential non-invasive biomarker for evaluating ischemic stroke in conjunction with [18F]FDG PET.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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