Fabrication of curcumin-incorporated human amniotic membrane extracellular matrix-derived scaffold to enhance full-thickness wound healing in diabetic rats.

The multifactorial nature of diabetic wounds necessitates a mixed approach for successful treatment. Compensation of degenerated wound tissue extracellular matrix (ECM) and application of anti-inflammatory and antioxidant agents have been shown to be promising. Here, an attempt was made to fabricate...

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Publicado en:Histochemistry & Cell Biology Vol. 163; no. 1; pp. 1 - 18
Autores principales: Moharrami Kasmaie, Farshad, Mehdinezhad Roshan, Mehdi, Nasiry, Davood, Abdollahifar, Mohammad Amin, Kazemi Ashtiani, Mohammad, Erfanian, Saeideh, Zarkesh, Ibrahim, Mazaheri Meybodi, Azadeh, Piryaei, Abbas
Formato: Journal Article
Publicado: Springer Nature 2025
Acceso en línea:Ver este registro en EBSCOhost
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      place: New York, New York
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        10.1007/s00418-024-02335-4
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        atl: Fabrication of curcumin-incorporated human amniotic membrane extracellular matrix-derived scaffold to enhance full-thickness wound healing in diabetic rats.
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          Moharrami Kasmaie, Farshad
          Mehdinezhad Roshan, Mehdi
          Nasiry, Davood
          Abdollahifar, Mohammad Amin
          Kazemi Ashtiani, Mohammad
          Erfanian, Saeideh
          Zarkesh, Ibrahim
          Mazaheri Meybodi, Azadeh
          Piryaei, Abbas
        affil: https://ror.org/034m2b326 Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, 1985717443, Tehran, Iran
      sug:
      ab: The multifactorial nature of diabetic wounds necessitates a mixed approach for successful treatment. Compensation of degenerated wound tissue extracellular matrix (ECM) and application of anti-inflammatory and antioxidant agents have been shown to be promising. Here, an attempt was made to fabricate a biocompatible wound dressing from curcumin-incorporated human amniotic membrane (HAM) ECM-derived scaffold to accelerate diabetic wound healing in rats. Therefore, after inducing diabetes, an excisional ischemic wound was created on rat skin, then treatments were administered for a period of 21 days. The main groups were the diabetic animals that received an engraftment of HAM scaffold (HAMS group) and the curcumin-incorporated HAMS (HAMS/β/C group). Evaluation at post-wounding days 7, 14, and 21 indicated that the parameters related to regeneration, including wound closure, volume of new epidermis and dermis, proliferating cells, fibroblasts, blood vessels, collagen deposition, and tensile strength, as well as transcripts of Vegf, bFgf, and Tgf-β genes of the healed wound in both HAMS and HAMS/β/C groups were considerably greater than those of the diabetic group. Conversely, the presence of inflammatory cells, i.e., neutrophils and macrophages, and the transcripts of Tnf-α and Il-1β showed a dramatic decrease in the treated groups relative to the diabetic group. Finally, compared to the HAMS group, considerable differences were found with the HAMS/β/C group in almost all evaluated parameters. Overall, these results suggest that using the complementary or synergistic effects of curcumin and HAMS could be a promising approach to improve diabetic wound healing.
      pubtype: Academic Journal
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    language: English
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