A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging.
Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo...
| Publicado en: | Histochemistry & Cell Biology Vol. 163; no. 1; pp. 1 - 16 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181403662&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181403662 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: 2025 vid: 163 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 181403662 10.1007/s00418-024-02338-1 181403662 ppf: 1 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: A novel tape-free sample preparation method for human osteochondral cryosections for high throughput hyperspectral imaging. aug: au: Fan, Xiwei Donose, Bogdan Jones, Michael W. M. Howard, Daryl Torniainen, Jari Bertling, Karl Guo, Xiao Kewish, Cameron M. Lee, Kah Meng Sun, Antonia Rujia Rakic, Aleksandar Crawford, Ross Afara, Isaac O. Prasadam, Indira affil: https://ror.org/053v2gh09 Department of Orthopaedic Surgery, The Second Xiangya Hospital of Central South University, Changsha, China sug: ab: Understanding the osteochondral junction, where non-mineralised cartilage and mineralised bone converge, is crucial for joint health. Current sample preparation techniques are insufficient for detailed spatial hyperspectral imaging analysis. Using the enhanced Kawamoto method, we used the super cryo embedding medium’s temperature-dependent properties to transfer high-quality tissue samples onto slides for spatial imaging analysis. We transferred osteochondral samples using a tape-free system and successfully tested them in hematoxylin and eosin (HE), Safranin-O, nanomechanical assessments and nano-Fourier transform infrared (FTIR) mapping. This protocol elucidates the structural and elemental gradients, mechanical characteristics and distinctive biochemical layering, making it a useful tool for analysing biochemical properties’ co-distribution in healthy and diseased situations. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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