Investigation of the pathogen-specific antibody response in periprosthetic joint infection.
Purpose: Periprosthetic joint infections (PJIs) are a very demanding complication of arthroplasty. Diagnosis of PJI and pathogen identification pose considerable challenges in clinical practice. We hypothesized that the pathogen-specific immune response to PJI reflects the infection process, provide...
| Publicado en: | Infection Vol. 52; no. 6; pp. 2325 - 2338 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181463200&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181463200 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Dec2024 vid: 52 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 181463200 177557335 181463200 181463200 10.1007/s15010-024-02285-y 181463200 ppf: 2325 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Investigation of the pathogen-specific antibody response in periprosthetic joint infection. aug: au: Janz, Viktor Rakow, Anastasia Schröder, Leonie Hofer, André Wiebe, Sergej Schoon, Janosch Weiss, Stefan Bröker, Barbara M. Wassilew, Georgi I. Raafat, Dina affil: https://ror.org/025vngs54 Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475, Greifswald, Germany sug: subj: Prosthesis-Related Infections Surgery Antibodies Immunology Bloodborne Pathogens Immunology Proteomics Reoperation Arthroplasty, Replacement Methods Human Prospective Studies Pilot Studies Preoperative Period Intraoperative Period Postoperative Period Blood Analysis Antibody Formation Staphylococcus Streptococcus ab: Purpose: Periprosthetic joint infections (PJIs) are a very demanding complication of arthroplasty. Diagnosis of PJI and pathogen identification pose considerable challenges in clinical practice. We hypothesized that the pathogen-specific immune response to PJI reflects the infection process, provides clinically relevant information on disease course, and has the potential to further optimize antimicrobial therapy. Methods: We conducted a prospective matched cohort pilot study with 13 patients undergoing two-stage septic revision arthroplasty (PJI patients) between 06/2020 and 06/2021, as well as 11 control patients undergoing one-stage aseptic revision arthroplasty (Non-PJI patients). Pre-, intra- and postoperative serum samples were collected at standardized time points. We developed a custom Luminex®-based quantitative bead-based suspension array (Infection Array; IA), and used it for simultaneous measurement of antibody specificities against 32 pathogens commonly associated with PJI in 267 serum samples. Results: The IA was able to trace the dynamics of the pathogen-specific humoral immune response in all patients against PJI-related pathogens, prominently coagulase-negative staphylococci and streptococci. Pathogen-specific serum antibody titers declined in 62% of PJI patients over the course of treatment, while no changes in antibody titers were observed in 82% of Non-PJI patients during this study. Our serological data strongly suggested that antibody signatures reflect an immune response to microbial invasion. Conclusion: Our results provide insights into the pathophysiology of PJI and information on the individual disease courses. The IA is therefore a promising and novel serological tool of high resolution for monitoring the immunoproteomic footprints of infectious pathogens in the course of PJI. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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