The application status of sequencing technology in global respiratory infectious disease diagnosis.
Next-generation sequencing (NGS) has revolutionized clinical microbiology, particularly in diagnosing respiratory infectious diseases and conducting epidemiological investigations. This narrative review summarizes conventional methods for routine respiratory infection diagnosis, including culture, s...
| Publicado en: | Infection Vol. 52; no. 6; pp. 2169 - 2182 |
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| Autores principales: | , , |
| Formato: | review tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2024
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181463231&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181463231 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Dec2024 vid: 52 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 181463231 179017586 181463231 181463231 10.1007/s15010-024-02360-4 181463231 ppf: 2169 ppct: 13 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: The application status of sequencing technology in global respiratory infectious disease diagnosis. aug: au: Chen, Jingyuan Qin, Zhen Jia, Zhongwei affil: https://ror.org/02v51f717 Department of Global Health, School of Public Health, Peking University, Beijing, China sug: subj: Respiratory Tract Infections Diagnosis Sequence Analysis Culture Techniques Microscopy Immunoassay Polymerase Chain Reaction Validity Time Factors Microbiota Evaluation Genes Evaluation Virulence Evaluation Bacteria Classification Fungi Classification DNA Blood Sequence Analysis Economics Sensitivity and Specificity ab: Next-generation sequencing (NGS) has revolutionized clinical microbiology, particularly in diagnosing respiratory infectious diseases and conducting epidemiological investigations. This narrative review summarizes conventional methods for routine respiratory infection diagnosis, including culture, smear microscopy, immunological assays, image techniques as well as polymerase chain reaction(PCR). In contrast to conventional methods, there is a new detection technology, sequencing technology, and here we mainly focus on the next-generation sequencing NGS, especially metagenomic NGS(mNGS). NGS offers significant advantages over traditional methods. Firstly, mNGS eliminates assumptions about pathogens, leading to faster and more accurate results, thus reducing diagnostic time. Secondly, it allows unbiased identification of known and novel pathogens, offering broad-spectrum coverage. Thirdly, mNGS not only identifies pathogens but also characterizes microbiomes, analyzes human host responses, and detects resistance genes and virulence factors. It can complement targeted sequencing for bacterial and fungal classification. Unlike traditional methods affected by antibiotics, mNGS is less influenced due to the extended survival of pathogen DNA in plasma, broadening its applicability. However, barriers to full integration into clinical practice persist, primarily due to cost constraints and limitations in sensitivity and turnaround time. Despite these challenges, ongoing advancements aim to improve cost-effectiveness and efficiency, making NGS a cornerstone technology for global respiratory infection diagnosis. pubtype: Academic Journal doctype: review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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