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Automated multimodal imaging of Caenorhabditis elegans behavior in multi-well plates.

Assays of behavior in model organisms play an important role in genetic screens, drug testing, and the elucidation of gene-behavior relationships. We have developed an automated, high-throughput imaging and analysis method for assaying behaviors of the nematode Caenorhabditis elegans. We use high-re...

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Published in:Genetics Vol. 228; no. 4; pp. 1 - 11
Main Authors: Ji, Hongfei, Chen, Dian, Fang-Yen, Christopher
Format: pictorial research tables/charts Journal Article
Published: Oxford University Press / USA Dec2024
Online Access:View this record in EBSCOhost
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      dt: Dec2024
      vid: 228
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      pub: Oxford University Press / USA
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        10.1093/genetics/iyae158
        181541214
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        atl: Automated multimodal imaging of Caenorhabditis elegans behavior in multi-well plates.
      aug:
        au:
          Ji, Hongfei
          Chen, Dian
          Fang-Yen, Christopher
        affil: Department of Biomedical Engineering, College of Engineering, The Ohio State University, Columbus, OH 43210, USA
      sug:
        subj:
          Caenorhabditis Elegans
          Diagnostic Imaging Methods
          Automation
          Biological Assay
          Cell Physiology
          Animal Studies
          Behavior, Animal
          Receptors, Serotonin
          Locomotion Physiology
          Models, Biological Utilization
          Genetic Screening
          High-Throughput Screening Assays
          Software Utilization
          Funding Source
      ab: Assays of behavior in model organisms play an important role in genetic screens, drug testing, and the elucidation of gene-behavior relationships. We have developed an automated, high-throughput imaging and analysis method for assaying behaviors of the nematode Caenorhabditis elegans. We use high-resolution optical imaging to longitudinally record the behaviors of 96 animals at a time in multi-well plates, and computer vision software to quantify the animals' locomotor activity, behavioral states, and egg-laying events. To demonstrate the capabilities of our system, we used it to examine the role of serotonin in C. elegans behavior. We found that egg-laying events are preceded by a period of reduced locomotion, and that this decline in movement requires serotonin signaling. In addition, we identified novel roles of serotonin receptors SER-1 and SER-7 in regulating the effects of serotonin on egg laying across roaming, dwelling, and quiescent locomotor states. Our system will be useful for performing genetic or chemical screens for modulators of behavior.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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