Bioinformatics Analysis and Spatiotemporal Distribution of the fliC Gene and Its Protein Isolated from Escherichia coli-Infected Patients in Eastern Algeria.
Background: The fliC locus in Escherichia coli primarily encodes flagellar (H) antigens. Exploring fliC sequence diversity will shed light on the mechanisms of bacterial pathogenicity. This study examined the presence of fliC mutant strains of E. coli in infected patients from different age groups,...
| Published in: | Malaysian Journal of Medical Sciences Vol. 31; no. 5; pp. 161 - 194 |
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| Main Authors: | , , |
| Format: | research tables/charts Journal Article |
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Malaysian Journal of Medical Sciences
2024
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181600225&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181600225 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1394195X YQP jtl: Malaysian Journal of Medical Sciences issn: 1394195X maglogo: N pubinfo: dt: 2024 vid: 31 iid: 5 pid: 23493 pub: Malaysian Journal of Medical Sciences artinfo: ui: 181600225 181600225 181600225 10.21315/mjms2024.31.5.12 181600225 ppf: 161 ppct: 33 formats: tig: atl: Bioinformatics Analysis and Spatiotemporal Distribution of the fliC Gene and Its Protein Isolated from Escherichia coli-Infected Patients in Eastern Algeria. aug: au: Harrar, Abdenassar Hamat, Rukman Awang Hamidechi, Mohamed Abdelhafid affil: Department of Microbiology, Faculty of Nature and Life Sciences, Frère Mentouri Constantine 1 University, Ain El-Bey, Algeria sug: subj: Escherichia Coli Infections Familial and Genetic Bacterial Proteins Analysis Mutation Evaluation Bioinformatics Evaluation Genetics, Microbial Human Funding Source Malaysia Male Female Child Adult Middle Age Aged Aged, 80 and Over Algeria Retrospective Design Record Review Cross Sectional Studies Descriptive Statistics Academic Medical Centers Sequence Analysis Genetics Data Analysis, Statistical Phylogenetics Genetic Algorithms Genetic Variation Signal Transduction Transferases Child: 6-12 years Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Background: The fliC locus in Escherichia coli primarily encodes flagellar (H) antigens. Exploring fliC sequence diversity will shed light on the mechanisms of bacterial pathogenicity. This study examined the presence of fliC mutant strains of E. coli in infected patients from different age groups, sexes and sample types in eastern Algerian provinces over a span of 2 years. Methods: This retrospective, cross-sectional study involved three provinces in eastern Algeria: i) Bordj Bou Arreridj, ii) Setif and iii) Batna. A total of 75 E. coli isolates were obtained from the University State Hospital Centre. Two types of analyses were conducted: i) a bioinformatics analysis of the protein sequences translated from the fliC genes, specifically the fliC flagellar sequences and ii) a multifactorial statistical analysis (multiple correspondence analysis [MCA]) of the population of infected patients, considering various parameters. The fliC protein sequences were aligned using the Multiple Alignment using Fast Fourier Transform (MAFFT) programme. The alignment results were then visualised using the MView programme. Finally, a phylogenetic tree was constructed using the maximum likelihood algorithm in MEGA 11 software. Results: Bioinformatics analysis highlighted the strong conservation of the structures of the fliC protein sequences, especially at the two N- and C-terminal ends, and strong variability in the central zone. This remarkable fliC intersequence similarity is corroborated by the presence of protein motifs identified in the PROSITE protein motif database. Conclusion: fliC mutations in E. coli were not detected in the clinical samples of patients from hospitals in the three Algerian Provinces. Our analysis revealed that all the samples exhibited characteristics of wild-type virulent bacteria without mutations. A multicentre study is warranted for epidemiological surveillance of fliC mutant strains for future preventive measures. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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