Isoquercitrin Inhibits Lung Cancer Cell Growth Through Triggering Pyroptosis and Ferroptosis.
Isoquercitrin possesses anti-tumor activity in several types of cancers, however, its effects and underlying mechanisms on lung cancer have not been reported. Human lung cancer cell lines as well as normal lung epithelial BEAS-2B cells were treated with isoquercitrin. The influences of isoquercitrin...
| Publicado en: | Nutrition & Cancer Vol. 77; no. 2; pp. 299 - 311 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=181909769&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 181909769 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: 2025 vid: 77 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 181909769 180355696 181909769 181909769 10.1080/01635581.2024.2416246 181909769 ppf: 299 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Isoquercitrin Inhibits Lung Cancer Cell Growth Through Triggering Pyroptosis and Ferroptosis. aug: au: Fan, Haiyin Xu, Pengfei Zou, Bin Wang, Huanyuan Li, Chao Huang, Jian affil: Department of Thoracic Surgery, Jiangxi Cancer Hospital, Nanchang, Jiangxi Province, China sug: subj: Lung Neoplasms Drug Therapy Lung Neoplasms Physiopathology Antineoplastic Agents Therapeutic Use Antineoplastic Agents Pharmacodynamics Quercetin Therapeutic Use Quercetin Pharmacodynamics Apoptosis Drug Effects Treatment Outcomes Animal Studies Mice Models, Biological In Vivo Studies Cell Proliferation Drug Effects Cell Viability Drug Effects Cell Line, Tumor Drug Effects Intracellular Signaling Peptides and Proteins Metabolism In Vitro Studies Quercetin Administration and Dosage Immunity Inflammation Reactive Oxygen Species Drug Effects Acetylcysteine Metabolism Signal Transduction Drug Effects Descriptive Statistics Data Analysis Software ab: Isoquercitrin possesses anti-tumor activity in several types of cancers, however, its effects and underlying mechanisms on lung cancer have not been reported. Human lung cancer cell lines as well as normal lung epithelial BEAS-2B cells were treated with isoquercitrin. The influences of isoquercitrin in vitro were evaluated by determining cell viability, apoptosis, pyroptosis, and ferroptosis. Additionally, A549 tumor-bearing mice were generated to explore the anti-cancer effect of isoquercitrin in vivo. We found that isoquercitrin dose-dependently reduced lung cancer cells' viability, with no toxicity against BEAS-2B cells. Isoquercitrin at 40 μM and 80 μM was used in vitro. Isoquercitrin increased apoptosis, elevated NLRP3 inflammasome activation-mediated pyroptosis, and promoted ferroptosis in lung cancer cells. NLRP3 knockdown and caspase-1 selective inhibitor VX-765 attenuated isoquercitrin-induced pyroptosis and ferroptosis, but not apoptosis. Furthermore, isoquercitrin accelerated ROS generation, while ROS inhibitor N-acetylcysteine abrogated isoquercitrin-induced apoptosis, NLRP3 related-pyroptosis and ferroptosis. In vivo, isoquercitrin (1 mg/kg and 5 mg/kg) inhibited tumor growth, increased apoptosis, NLRP3-related pyroptosis, ferroptosis and ROS generation in tumors. Taken together, isoquercitrin inhibits lung cancer growth by triggering ROS/NLRP3-mediated pyroptosis and ferroptosis, with ROS also directly inducing apoptosis. This suggests that isoquercitrin might be a potential therapeutic agent for lung cancer. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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