A low-dose prebiotic fiber supplement reduces lipopolysaccharide-binding protein concentrations in a subgroup of young, healthy adults consuming low-fiber diets.

• Fiber decreased lipopolysaccharide-binding protein (LBP) in those with high LBP • LBP was negatively correlated with microbial genes for antibiotic synthesis • LBP was positively correlated with sulfated molecules like perfluorooctanesulfonate Although the beneficial effects of fiber supplementati...

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Publicado en:Nutrition Research Vol. 133; pp. 138 - 148
Autores principales: Romo, Eduardo Z., Hong, Brian V., Agus, Joanne K., Jin, Yanshan, Kang, Jea Woo, Zivkovic, Angela M.
Formato: research Journal Article
Publicado: Elsevier B.V. Jan2025
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jan2025
      vid: 133
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      pub: Elsevier B.V.
      place: New York, New York
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        182095568
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        10.1016/j.nutres.2024.11.013
        182095568
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        atl: A low-dose prebiotic fiber supplement reduces lipopolysaccharide-binding protein concentrations in a subgroup of young, healthy adults consuming low-fiber diets.
      aug:
        au:
          Romo, Eduardo Z.
          Hong, Brian V.
          Agus, Joanne K.
          Jin, Yanshan
          Kang, Jea Woo
          Zivkovic, Angela M.
        affil: Department of Nutrition, University of California, Davis, CA, USA
      sug:
        subj:
          Prebiotics Administration and Dosage
          Dietary Fiber Administration and Dosage
          Dietary Supplementation
          Lipopolysaccharides Blood
          Lipopolysaccharide-Binding Protein Analysis
          Human
          Male
          Female
          Young Adult
          Double-Blind Studies
          Crossover Design
          Descriptive Statistics
          Exploratory Research
          Biological Markers
          Phosphatidylcholines
          Lipoproteins, HDL Cholesterol
          Acyltransferases
          Bile Acids and Salts
          Enzyme-Linked Immunosorbent Assay
          Environmental Exposure
          Male
          Female
      ab: • Fiber decreased lipopolysaccharide-binding protein (LBP) in those with high LBP • LBP was negatively correlated with microbial genes for antibiotic synthesis • LBP was positively correlated with sulfated molecules like perfluorooctanesulfonate Although the beneficial effects of fiber supplementation on overall health and the gut microbiome are well-known, it is not clear whether fiber supplementation can also alter the concentrations of lipopolysaccharide-binding protein (LBP), a marker of intestinal permeability. A secondary analysis of a previously conducted study was performed. In the randomized-order, placebo-controlled, double-blinded, cross-over study 20 healthy, young participants consuming a low-fiber diet at baseline were administered a daily dose of 12 g of prebiotic fiber compared with a placebo over a period of 4 weeks with a 4-week washout between arms. In this secondary analysis, we hypothesized that the fiber supplement would reduce LBP concentration. We further hypothesized that lecithin cholesterol acyltransferase activity, a measure of high-density lipoprotein functional capacity, would be altered. Fiber supplementation did not significantly alter LBP concentration or lecithin cholesterol acyltransferase activity in the overall cohort. However, in a subgroup of individuals with elevated baseline LBP concentrations, fiber supplementation significantly reduced LBP from 9.27 ± 3.52 to 7.02 ± 2.32 µg/mL (P =.003). Exploratory analyses found positive correlations between microbial genes involved in lipopolysaccharide synthesis and conversely negative correlations with genes involved in antibiotic synthesis and LBP. Positive correlations between LBP and multiple sulfated molecules including sulfated bile acids and perfluorooctanesulfonate, and ibuprofen metabolites were also found. These findings highlight multiple environmental and lifestyle factors such as exposure to industrial chemicals and medication intake, in addition to diet, which may influence the association between the gut microbiome and gut barrier function. The study involved 20 healthy, young adults participating in a double-blinded, randomized-order, placebo-controlled intervention study. Participants were randomized to either first receive a prebiotic fiber or a placebo over a 4-week treatment period, followed by a 4-week washout phase, and then crossed over to the alternate treatment. The impact of the intervention on LBP concentrations was assessed using a 96-well ELISA kit. Participants were also stratified by those with above- and below-median LBP concentrations at baseline. A significant reduction in LBP (P =.020) was found after the prebiotic fiber intervention arm in those individuals who had above-median LBP concentrations at baseline. Abbreviations: ELISA, enzyme-linked immunosorbent assay; LBP, lipopolysaccharide-binding protein. [Display omitted]
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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