Leveraging a new data resource to define the response of Cryptococcus neoformans to environmental signals.
Cryptococcus neoformans is an opportunistic fungal pathogen with a polysaccharide capsule that becomes greatly enlarged in the mammalian host and during in vitro growth under host-like conditions. To understand how individual environmental signals affect capsule size and gene expression, we grew cel...
| Publicado en: | Genetics Vol. 229; no. 1; pp. 1 - 30 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Jan2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=182166170&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 182166170 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Jan2025 vid: 229 iid: 1 pid: 622 pub: Oxford University Press / USA artinfo: ui: 182166170 182166170 182166170 10.1093/genetics/iyae178 182166170 ppf: 1 ppct: 29 formats: tig: atl: Leveraging a new data resource to define the response of Cryptococcus neoformans to environmental signals. aug: au: Kang, Yu Sung Jung, Jeffery Brown, Holly L Mateusiak, Chase Doering, Tamara L Brent, Michael R affil: Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St. Louis, MO 63110, USA sug: subj: Cryptococcus Gene Expression Cyclic AMP Metabolism Fungi In Vitro Studies Culture Media Cell Culture Techniques Sequence Analysis RNA Funding Source ab: Cryptococcus neoformans is an opportunistic fungal pathogen with a polysaccharide capsule that becomes greatly enlarged in the mammalian host and during in vitro growth under host-like conditions. To understand how individual environmental signals affect capsule size and gene expression, we grew cells in all combinations of 5 signals implicated in capsule size and systematically measured cell and capsule sizes. We also sampled these cultures over time and performed RNA-seq in quadruplicate, yielding 881 RNA-seq samples. Analysis of the resulting data sets showed that capsule induction in tissue culture medium, typically used to represent host-like conditions, requires the presence of either CO2 or exogenous cyclic AMP. Surprisingly, adding either of these pushes overall gene expression in the opposite direction from tissue culture media alone, even though both are required for capsule development. Another unexpected finding was that rich medium blocks capsule growth completely. Statistical analysis further revealed many genes whose expression is associated with capsule thickness; deletion of one of these significantly reduced capsule size. Beyond illuminating capsule induction, our massive, uniformly collected data set will be a significant resource for the research community. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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