Towards the clinical translation of a silver sulfide nanoparticle contrast agent: large scale production with a highly parallelized microfluidic chip.
Purpose: Ultrasmall silver sulfide nanoparticles (Ag2S-NP) have been identified as promising contrast agents for a number of modalities and in particular for dual-energy mammography. These Ag2S-NP have demonstrated marked advantages over clinically available agents with the ability to generate highe...
| Publicado en: | European Journal of Nuclear Medicine & Molecular Imaging Vol. 52; no. 3; pp. 1177 - 1189 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Feb2025
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=182409452&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 182409452 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16197070 NPC jtl: European Journal of Nuclear Medicine & Molecular Imaging issn: 16197070 maglogo: N pubinfo: dt: Feb2025 vid: 52 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 182409452 180794002 10.1007/s00259-024-06967-5 182409452 ppf: 1177 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Towards the clinical translation of a silver sulfide nanoparticle contrast agent: large scale production with a highly parallelized microfluidic chip. aug: au: Mossburg, Katherine J. Shepherd, Sarah J. Barragan, Diego O, Nathaniel H. Berkow, Emily K. Maidment, Portia S. N. Rosario Berrios, Derick N. Hsu, Jessica C. Siedlik, Michael J. Yadavali, Sagar Mitchell, Michael J. Issadore, David Cormode, David P. affil: https://ror.org/00b30xv10 Department of Bioengineering, University of Pennsylvania, Philadelphia, PA, USA sug: ab: Purpose: Ultrasmall silver sulfide nanoparticles (Ag2S-NP) have been identified as promising contrast agents for a number of modalities and in particular for dual-energy mammography. These Ag2S-NP have demonstrated marked advantages over clinically available agents with the ability to generate higher contrast with high biocompatibility. However, current synthesis methods for inorganic nanoparticles are low-throughput and highly time-intensive, limiting the possibility of large animal studies or eventual clinical use of this potential imaging agent. Methods: We herein report the use of a scalable silicon microfluidic system (SSMS) for the large-scale synthesis of Ag2S-NP. Ag2S-NP produced using this system were compared to bulk synthesis and a commercially available microfluidic device through characterization, contrast generation, in vivo imaging, and clearance profiles. Results: Using SSMS chips with 1 channel, 10 parallelized channels, and 256 parallelized channels, we determined that the Ag2S-NP produced were of similar quality as measured by core size, concentration, UV–visible spectrometry, and in vitro contrast generation. Moreover, by combining parallelized chips with increasing reagent concentration, we were able to increase output by an overall factor of 5,100. We also found that in vivo imaging contrast generation was consistent across synthesis methods and confirmed renal clearance of the ultrasmall nanoparticles. Finally, we found best-in-class clearance of the Ag2S-NP occurred within 24 h. Conclusions: These studies have identified a promising method for the large-scale production of Ag2S-NP, paving the way for eventual clinical translation. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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